中文摘要：

为鉴定禽流感病毒（AIV）感染状态下的差异表达蛋白,本研究将H5N1亚型AIV感染组与对照组小鼠肺组织蛋白样品分别进行双向电泳分离,并采用ImageMaster 2D Platinum 6.0软件进行分析。在AIV感染72h后,共有9个表达差异显著的蛋白点（ratio〉2,p〈0.05）,而且该蛋白在感染组均呈上调表达。将差异蛋白进行串联质谱分析,并对其中7个蛋白进行鉴定,包括：interferon-induced protein with tetratricopeptide repeats 3（IFIT-3）、ATP依赖的干扰素反应蛋白1（ADIR1）、γ干扰素诱导的p47鸟苷三磷酸酶（IRG）、T细胞受体a TA27、气味受体S86、胞苷单磷酸激酶2（Cmpk2）和肌球蛋白。将前3个蛋白基因采用荧光定量PCR方法进行mRNA水平的验证,所获得的结果与双向电泳结果一致。本研究为在蛋白质组水平进一步分析H5N1亚型AIV与宿主相互作用奠定了基础。

英文摘要：

In this study, the differentially expressed proteins were investigated in H5N1 avian influenza virus （AIV） infected mice by two dimensional gel electrophoresis （2-DE）. The lung tissue lysis from the AIV infected or control groups were extracted at 72 hours post-inoculation and separated by 2-DE. Nine protein spots were found to have notably differential expression between the 2 groups （ratio2, p0.05）, up-regulated in the AIV infected mice. Seven of them were identified by MALDI-TOF/TOF, including ATP-dependent interferon response protein1 （ADIR1）, interferon-induced protein with tetratricopeptide repeats 3 （IFIT-3）, T cell receptor alpha TA27, Interferon-gamma-inducible p47 GTPase （IRG）, Cytidine monophosphate kinase 2 （Cmpk2 protein）, odorant receptor S86, myosin light chain 1/3. Moreover, the transcriptional levels of 3 genes （ADIR, IFIT-3, and IRG） were verified by real-time PCR, which was completely consistent with the proteomic analysis result. Our data could provide an insight into the AIV-host interactions.