中文摘要：

瞄准：调查乳杆菌 plantarum 的机制(L。plantarum ) 在在老鼠的外科手术前、手术后的试验性的妨碍的黄疸的内脏障碍上的行动。方法：四十只老鼠随机被划分成一些假冒操作，胆汁管结扎(BDL ) ， BDL + L。plantarum， BDL + 内部胆汁的排水(IBD ) ，和 BDL +IBD + L。plantarum。在 L 以后的十天。plantarum 管理，血和 ileal 样品为词法检查，和肠的障碍从老鼠被收集功能，肝功能，肠的氧化压力和蛋白质 kinase C (PKC ) 活动测量。PKC 和紧密的连接(TJ ) 的分发和表示蛋白质例如 occludin， zonula occludens-1， claudin-1， claudin-4，连接粘附分子 -- A 和 F 肌动朊，被扫描显微镜学的共焦的激光检验， immunohistochemistry，西方的弄污，即时荧光灯量的聚合酶链反应试金。结果：L。plantarum 管理实质地恢复了内脏障碍，减少的 enterocyte apoptosis，改进肠的氧化应力，支持了蛋白质 kinase 的活动和表示(BDL 对 BDL + L。plantarum， 0.295 l 纸巾在石蜡和 sectioned 被嵌入。(hematoxylin 和曙红和 Toluidine 蓝色) 组织学的染色被执行表明 HCC 的发作和 sulphated glycosaminoglycan (sGAG ) 的内容。染色的 Immunohistochemical 被执行调查 chondroitin 的表示硫酸盐(CS )/dermatan 硫酸盐(DS )-GAG, heparan 硫酸盐(HS )-GAG, keratan 硫酸盐(KS ) 在肝纸巾作呕。而且，包括在肝纸巾的 aggrecan， versican， biglycan 和 decorin， CSPG 家庭成员的表示和分发也是 immunohistochemically 坚定的。结果：在兽穴的 16 wk 管理以后，恶意的小瘤是 observ 吗？

英文摘要：

AIM：To investigate the mechanisms of Lactobacillus plantarum（L.plantarum）action on gut barrier in preoperative and postoperative experimental obstructive jaundice in rats.METHODS：Forty rats were randomly divided into groups of sham-operation,bile duct ligation（BDL）,BDL ＋L.plantarum,BDL＋internal biliary drainage（IBD）,and BDL＋IBD＋L.plantarum.Ten days after L.plantarum administration,blood and ileal samples were collected from the rats for morphological examination,and intestinal barrier function,liver function,intestinal oxidative stress and protein kinase C（PKC）activity measurement.The distribution and expression of the PKC and tight junction（TJ）proteins,such as occludin,zonula occludens-1,claudin-1,claudin-4,junction adhesion molecule-A and F-actin,were examined by confocal laser scanning microscopy,immunohistochemistry,Western blotting,real-time fluorescent quantitative polymerase chain reaction assay.RESULTS：L.plantarum administration substantially restored gut barrier,decreased enterocyte apoptosis,improved intestinal oxidative stress,promoted the activity and expression of protein kinase（BDL vs BDL＋L.plantarum,0.295±0.007 vs 0.349±0.003,P〈0.05;BDL＋IBD vs BDL＋IBD＋L.plantarum,0.407±0.046 vs 0.465±0.135,P〈0.05）,and particularly enhanced the expression and phosphorylation of TJ proteins in the experimental obstructive jaundice（BDL vs BDL＋L.plantarum,0.266±0.118 vs 0.326±0.009,P〈0.05）.The protective effect of L.plantarum was more prominent after internal biliary drainage（BDL＋IBD vs BDL ＋IBD＋L.plantarum,0.415±0.105 vs 0.494±0.145,P〈0.05）.CONCLUSION：L.plantarum can decrease intestinal epithelial cell apoptosis,reduce oxidative stress,and prevent TJ disruption in biliary obstruction by activating the PKC pathway.