中文摘要：

目的探讨骨髓间充质干细胞（bone mesenchymal stem cell,BMSC）移植对局灶性脑缺血大鼠缺血皮质神经元核抗原（neuronal nuclei,NeuN）和神经元素l（neurogeninl，Ngnl）的影响。方法64只健康雄性成年Sprague—Dawley大鼠随机分为正常对照（normal,N）±磷酸盐缓冲液（phosphate buffered solution，PBS）组、大脑中动脉闭塞（middle cerebral artery occlusion，MCAO）±PBS组、正常对照＋BMSC组和MCAO＋BMSC组，每组16只。线栓法制作大鼠MCAO模型。体外培养BMSC，在模型制作后24h进行脑内移植。采用MRI活体检测脑梗死体积，NeuN／DAPI和Ngn1／DAPI免疫荧光双标法以及蛋白印迹法检测缺血周围脑组织NeuN和Ngnl表达。结果移植后14d，MCAO组大鼠T1和T2加权成像均可见大脑皮质和纹状体存在异常信号区，MCAO＋BMSC组脑梗死体积显著性小于MCAO＋PBS组（32．5％±4．2％对47．9％±7．9％；P〈0．001）。免疫荧光双标染色显示，MCAO±PBS组NeuN^＋／DAPI^＋[（976．2±87．5）个／mm^2对（1908．3±127．8）个／mm^2；P〈0．01]和Ngn1^＋／DAPI^＋[（251．6±23．1）个／mm^2对（285．1±25．2）个／mm^3；P〈0．01]细胞数量均显著性少于N±PBS组，但MCAO±BMSC组NeuN’／DAPI^＋[（1439．9±101．7）个／mm^2；P〈0．01]和Ngn1^＋／DAPI^＋[（356．3±35．6）个／mm^2；P〈0．01]显著性多于MCAO±PBS组。蛋白质印迹分析显示，MCAO±PBS组NeuN（0．69±0．06对0．91±0．09；P〈0．01）和Ngn1（0．53±0．05对0．62±0．07；P〈0．01）蛋白表达水平显著性低于N＋PBS组，但MCAO±BMSC组NeuN（0．82±0．07；P〈0．01）和Ngn1（0．77±0．09；P〈0．01）蛋白表达水平显著性高于MCAO＋PBS组。结论BMSC移植可促进NeuN和Ngnl表达，减轻MCAO脑损伤。

英文摘要：

detect the expression of NeuN and Ngnl around ischemic brain tissue. Results On day 14 after transplantation, the T1- and T2-weighted imaging revealed that the cerebral cortex and striatum had abnormal signal areas in the rats of the MCAO group. The infarct volume of the MCAO ＋ BMSC group was significantly less than that of the MCAO ＋ PBS group （32. 5% ± 4. 2% vs. 47. 9% ± 7.9% ; P 〈 0. 01 ）. Immunofluorescence double- labeling assay showed that the numbers of cells of NeuN＋/DAPI＋ （976.2 ± 87. 5/mm： vs. 1 908. 3 ± 127. 8/mm2; P〈 0.01） and Ngnl ＋/DAPI＋ （251.6± 23.1/mm2 vs. 285. 1 ±25.2/mm^2; P〈 0.01） of the MCAO ＋PBS group were significantly less than those of the N＋ PBS group, but those of NeuN＋/DAPI＋ （1 439. 9 ± 101.7/mm^2; P 〈0. 01） and Ngnl ＋/DAPI ＋ （356. 3 ± 35. 6/mm2; p 〈 0. 01 ） of the MCAO ＋ BMSC group were significantly more than the MCAO ＋ PBS group. Western blot analysis showed that the protein expression levels of NeuN （0. 69 ± 0. 06 vs. 0. 91± 0. 09; P 〈 0. 01） and Ngnl （0. 53 ± 0. 05 vs. 0. 62 ± 0. 07; P 〈 0. 01 ） of the MCAO ＋ PBS group were signific antly lower than those of the N ＋ PBS group, but those of NeuN （0. 82± 0. 07; P 〈 0. 01 ） and Ngnl （0. 77 ±0. 09; P 〈 0. 01 ） of the MCAO ＋ BMSC group were significantly higher than the MCAO ＋ PBS group. Conclusions BMSC transplantation may promote the expression of NeuN and Ngnl, and alleviate MCAO caused brain injury.