中文摘要：

目的探讨大鼠骨髓间充质干细胞（BMSCs）移植对局灶性脑缺血大鼠内源性神经干细胞（NSCs）分化的影响，为BMSCs移植治疗局灶性脑缺血性疾病提供科学的理论依据。方法采用完全随机法将40只Sprague—Dawley大鼠分为正常对照（CON）组、大脑中动脉阻塞模型（MCAO）组、BMSCs移植组与磷酸盐缓冲液（PBS）组。贴壁法体外培养大鼠BMSCs，采用线栓法制成大鼠局灶性脑缺血模型，造模后24h侧脑室移植BMSCs，腹腔注射5-bromo-2＇-deoxyuridille（BrdU）标记增殖细胞。移植后28d，采用BrdU／β-tubulin与BrdU／GFAP免疫荧光双标法检测BMSCs移植对内源性NSCs分化的影响，并采用尼氏染色法观察各组大鼠损伤侧脑组织神经元的变化。结果移植后28d，MCAO组可见少量BrdU＋β—tubulin＋细胞，显著少于CON组（P〈0．01），PBS组与MCAO纽BrdU＋β-tubulin＋细胞差异无显著性（P〉0．05），BMSCs移植组可见大量BrdU＋β-tubulin＋细胞，显著高于MCAO组（P〈0．01）与PBS组（P〈0．01）；MCAO组可见大量BrdU＋GFAP＋细胞，显著高于CON组（P〈0．D1），PBS组与MCAO组BrdU＋β-tubulin＋细胞差异无显著性（P〉0．05），BMSCs移植组可见少量BrdU＋GFAP＋细胞，显著低于MCAO组（P〈0．01）与PBS组（P〈0．01）。MCAO组大鼠损伤侧大脑皮层神经元细胞数明显少于CON组（P〈0．01），PBS组与MCAO组差别不显著（P〉0．05）；BMSCs移植组大鼠大脑皮层神经元细胞数显著多于MCAO组，差异具有统计学意义（P〈0．01）。，结论BMSCs可促进局灶性脑缺血大鼠内源性NSCs分化成为成熟的神经元与星形胶质细胞，修复脑损伤。

英文摘要：

Objective To exph,re the efl＇eets of bone marrow mesencbymal stem cells（BMSCs） on the differentiation of endoge- nous neural stem cells （NSCs） in ischemie rats,thus to provide theoretical basis for the application of BMSCs in focal cerebral ischemia disea- ses. Methods Forty Sprague-1）awley rals were divided into normal control group （ CON ） , middle cerebral artery occlusion model （ MCAO ） group, BMSCs transplantation group and phusphale buffer solution（PBS） group randomly. The BMSCs were cuhured by adhesion method,the focal brain ischemia models were made by thread ligation method. 24 h after isehemia,the BMSCs were transplanted into the brain via lateral ventricle. 5-bromo-2＇-deoxyuridine（BrdU） were intraperitoneally injected to label the proliferating ceils. Twenty-eight days ＇alter transplanta- lion,the newborn neurons and astrocytes were examined by BrdU/ [3 -tubulin and BrdU/GFAP immunofluorescenee double labeling method, and Nissl staining was used to observe changes of neurons in each glvup. Results Twenty-eight days at]er transplantation,there were less Br dU ＋ β -tubulin ＋ cells in the MCAO group as cumpared with the CON group（ P 〈 0.01 ）. There was no significant difference in the BrdU ＋ β -tubulin ＋ cells between the PBS trod MCA（.） group（ P 〉 0.05 ）. More BrdU＇ β-tubulin ＋ cells were observed in the BMSCs group as comparedwith the MCAO （ P 〈 0.01 ） and PBS group （ P 〈 0.01 ）. There were more BrdU ＋ GFAP ＋ cells in the MCAO group as compared with the CON group （ P 〈 0.01 J. And there was no significant difference in the BrdU ＋ GFAP ＋ cells between the PBS and MCAO group （ P 〉 0.05 ）. Less Br dU ＋ GFAP ＋ cells were observed in the BMSCs group as compared with the MCAO（P 〈 0.01） and PBS group（P 〈 0.01）. There were less contact neurons in the MCAO group than those in CON group（ P 〈0.01 ）. And there was no significant difference in the number of neurons between PBS and MCAO group （ P 〉 0