中文摘要：

为检测伪狂犬病病毒（PRV）在体外细胞中糖蛋白H（gH）的表达情况,本研究构建原核表达重组质粒pET-gHN660,并在大肠杆菌中诱导表达重组蛋白,纯化的gH重组蛋白免疫实验动物制备抗PRVgH抗体,经westernblot和IFA检测到病毒感染细胞中gH蛋白的表达,病毒感染细胞后表达的gH蛋白大小为95ku,定位于细胞浆中,gH蛋白在病毒感染细胞4h可以检出,随PRV的复制gH蛋白表达增加,gH蛋白可以作为PRV复制的指示蛋白。本研究利用制备的抗体分析感染细胞中gH蛋白的表达情况,初步探讨感染细胞中PRV的复制,为PRV和宿主相互作用的研究奠定基础。

英文摘要：

To analysis the expression of glycoprotein H （gH） in pseudorabies virus （PRV） infected cell, partial domain of PRV gH was expressed in E. coli and purified to prepare the anti-PRV gH antibodies in rabbit. gH protein expressed in PRV infected cell were detected by western blot and immunofluorescence. The results showed that the molecular weight of PRV gH was 95 ku and expressed at 4 hours post infection, mainly located in the cytoplasm, and accumulated as PRV replication, PRV gH would be a potential indicator protein of PRV replication in infected cells. The expression of PRV gH were analysised using anti-PRV gH antibodies, and the PRV replication was discussed.