中文摘要：

目的：鉴定肿瘤转移相关基因1（MTA1）的HLA-A3限制性细胞毒性T淋巴细胞（CTL）表位。方法：首先运用RT-PCR方法检测MTA1mRNA在肿瘤细胞系EC-1、EC-109和T-47D中的表达情况。然后通过BIMAS、SY-FPEITHI、NetCTL1.2及IEDB4个软件预测筛选MTA1HLA-A3限制性的候选表位。候选表位通过标准Fmoc化学法合成,通过结合力实验检测表位与T2A3细胞表面HLA-A3分子的结合力水平,通过体外细胞毒实验检测候选肽诱导CTL的能力。结果：MTA1mRNA在EC-1、EC-109和T-47D细胞中均有表达。候选表位P130与HLA-A3分子有弱结合力,P294、P559与HLA-A3分子有中等结合力。细胞毒实验结果显示多肽P130、P294和P559对EC-1细胞均有一定的杀伤作用（F细胞=176.107,F效靶比=48.306,F交互=35.686,P均〈0.001）。结论：多肽P130、P294、P559能够诱导体外抗肿瘤免疫反应,可能成为新的抗肿瘤多肽疫苗的候选表位。

英文摘要：

Aim：To identify the HLA-A3 restricted cytotoxic T lymphocyte（CTL） epitopes from metastasis associated gene 1（MTA1）.Methods：RT-PCR was used to determine the expression of MTA1 mRNA in cancer cell lines,EC-1,EC-109 and T-47D.HLA-A3 epitopes from MTA1 protein were predicted by BIMAS,SYFPEITHI,NetCTL 1.2 and IEDB.Peptides were synthesized by standard Fmoc chemistry method.Their binding affinities towards HLA-A3 molecule were evaluated by T2A3 cells binding assay.Their ability to induce T cell response was investigated by using cytotoxicity assay in vitro.Results：The expression of MTA1 mRNA was observed in EC-1,EC-109 and T-47D cells.The candidate peptide P130 showed weak affinity towards HLA-A3 molecule,while P294 and P559 showed moderate affinity.The CTLs induced by all the three peptides could lyse EC-1 cells（Fcell=176.107,Fratio=48.306,Finteraction=35.686,P0.001）.Conclusion：The peptides P130,P294,and P559 could induce anti-tumor inmmunity in vitro and serve as candidates towards antitumor peptide vaccines.