中文摘要：

四环素类抗生素是目前世界上应用最为广泛的抗生素之一,也是我国畜禽养殖业中使用量最大的兽药和饲料添加剂.由于该类物质不易被生物体吸收且当前的污水处理设施对抗生素不能彻底去除,故大量抗生素及其代谢产物最终进入地表水环境,其对水生态系统和人体健康的潜在威胁值得关注.本研究以水生态系统初级生产者淡水绿藻为受试生物,系统考察四环素、金霉素和强力霉素对蛋白核小球藻、斜生栅藻细胞膜通透性和生长抑制作用的影响.结果表明,抗生素暴露96 h后,强力霉素在整个作用浓度范围内使供试淡水绿藻细胞膜通透性降低;而金霉素和四环素低浓度暴露可以增大藻细胞膜通透性,此后随着作用浓度的增大转为降低.3种抗生素对淡水绿藻生长抑制率大致呈现强力霉素〉四环素〉金霉素的顺序趋势.斜生栅藻对四环素类抗生素的毒性响应较蛋白核小球藻更为敏感.

英文摘要：

Tetracycline antibiotics are a group of antibiotics which has been extensively used in most countries and are also the most widely used veterinary drugs and food additives in aquaculture and livestock industries of China.Because the substances are poorly adsorbed in the gut of animals and are only partially eliminated in sewage treatment plants,the antibiotics and their metabolites reach surface water where they may affect the aquatic organisms and human health.Herein,using green algae as test organisms,we studied the effects of tetracycline（TC）,chlortetracycline（CTC） and deoxyteracycline（DC） on the cell permeability and growth inhibition of chlorella pyrenoidosa and scenedesmus obliquus.After 96 h of antibiotics exposure,the permeability of the cell membranes of both algae was decreased in the entire concentration range of doxycycline and was increased after exposure to a low concentration of tetracycline and chlortetracycline,then was decreased with the increase of the two antibiotics concentrations.The growth inhibiting effects of these three antibiotics on chlorella pyrenoidosa and scenedesmus obliquus were in an order of DC TC CTC.The ecotoxic response of scenedesmus obliquus to tetracycline antibiotics was more sensitive than chlorella pyrenoidosa.