采用OECD标准滤纸接触法和人工土壤法,研究了3种离子液体1-丁基-3-甲基咪唑氯盐([Bmim]Cl)、1-己基-3-甲基咪唑氯盐([Hmim]Cl)、1-辛基-3-甲基咪唑氯盐([Omim]Cl)对赤子爱胜蚓(Eisenia fetida)的急性致死作用,并测定亚慢性暴露试验下蚯蚓的体重变化.滤纸接触法测得[Bmim]Cl、[Hmim]Cl、[Omim]Cl对蚯蚓24 h的半致死浓度(LC50)分别为109.60、50.38、7.94μg.cm-2;48 h的LC50分别为98.52、39.14、3.61μg.cm-2.人工土壤法得到[Bmim]Cl、[Hmim]Cl、[Omim]Cl对蚯蚓7 d的LC50分别为447.78、245.56、180.51 mg.kg-1,14 d的LC50分别288.42、179.75、150.35 mg.kg-1.蚯蚓在3种离子液体作用下表现出不同的中毒症状.3种离子液体对蚯蚓的生长表现出不同程度的抑制作用,体重随着浓度增加而下降.离子液体对蚯蚓毒性随着碳链长度的增加而增大.
Standard contact filter paper test of OECD and artificial soil test were used to study the acute lethal effect of three imidazolium chloride ionic liquids,1-butyl-3-methylimidazolium chloride(Cl),1-hexyl-3-methylimidazolium chloride(Cl),and 1-octyl-3-methylimidazolium chloride(Cl) on earthworm(Eisenia fetida),and the weight of the earthworms was measured after subtle exposure.The 24 h-LC50 values of Cl,Cl and Cl using the contact filter paper method were 109.60,50.38 and 7.94 μg·cm-2,respectively.The 48 h-LC50 values were 98.52,39.14 and 3.61 μg·cm-2,respectively.Using the artificial soil method,the 7 d-LC50values of Cl,Cl and Cl were 447.78,245.56 and 180.51 mg·kg-1,respectively,and the 14 d-LC50values were 288.42,179.75,150.35 mg·kg-1,respectively.There were differences in poisoning symptoms of the three ionic liquids on earthworms.The growth of Eisenia fetida was inhibited and declined with increasing ionic liquid concentration.The toxicity of ionic liquids on Eisenia fetida increased with the length of carbon chain.