中文摘要：

利用少根根霉（Rhizopus arrhizus，RH-07—13）直接发酵生木薯粉生产富马酸。探讨了摇瓶发酵中，不同的接种量和装液量对生产富马酸的影响，检测了发酵培养过程中的富马酸、葡萄糖、乙醇的变化以及发酵结束后的菌体生物量。结果表明，最佳的接种量为20％（体积分数），最佳的装液量为25mL／250mL。在此条件下，发酵168h，富马酸产量达49．88g／L，生物量达43．8g／L。

英文摘要：

The direct production of fumaric acid from raw tapioca flour by a strain of Rhizopus arrhizus RH-07-13 has been investigated. The effects of varying the liquid volume and inoculum concentration on fumaric acid produc- tion were investigated. Fumaric aeid, glucose and ethanol concentrations during fermentation and the biomass a- mount after fermentation were recorded. The maximum yield of fumaric acid of 49.88 g/L and biomass production of 43.8 g/L were obtained using the optimal inoeulum concentration of 20% and the optimal liquid volume of 25 mL/250 mL.