中文摘要：

目的：建立一种可用于中药血清药理研究的器官型脑片方法。方法：大鼠灌胃通心络1周获得含药血清,制备P3 SD乳鼠全脑器官型脑片,分别给予不同剂量通心络含药血清干预,通过倒置显微镜和免疫荧光染色观察含药血清对器官型脑片的影响。结果：培养1d、3d、7d时室下区、海马齿状回单位区域出现nestin＋新生细胞,数量均依大剂量含药血清组、小剂量含药血清组、对照血清组、空白组顺序递减,各组间新生细胞密度存在显著性差异。结论：器官型脑片简单、直观、能更好的模拟体内环境及控制实验条件,是一种可用于中药血清药理研究的优良方法。

英文摘要：

Objective： To establish a method, organotypie brain slice, for study on Chinese traditional medicine serum pharmacology. Methods：Sera containing Tongxiuluo were separated from SD rats administered Tongxiuluo for 1 week. Brain slices （300um） from postnatal 3rd day SD rats were prepared and administered different dose of Tongxiuluo sera or normal saline, then they were observed through invert microscope or detected by immunofluoreseence staining. Results： Neonatal cells labeled with nestin were found in the subventrical zone and hippecampal dentate gyrus when brain slices were being cultured for 1 day, 3 days and 7 days. The number of neonatal cells decreased in the order of high-dose Tongxinluo group, low-dose Tongxiuluo group, control serum group and normal saline group （ p 〈 0. 01 ）. Conclusion： Organotypic brain slice was a simple intuitionistic method for study on Chinese traditional medicine pharmacology, and it could mimic in vivo niche and control experimental condition better.