中文摘要：

用荧光光谱法在298K研究了Tris-HCl缓冲溶液（pH=7.1）中系列N-烷基-N,N-二（2-羟乙基）-N-甲基溴化铵（烷基链长为C12到C16）与牛血清白蛋白（BSA）的结合作用,考察了表面活性剂结构、BSA浓度对结合作用的影响,分别用Stern-Volmer方程、虚拟结合常数模型探讨了表面活性剂在浓度较低区域与BSA的作用机制.结果表明：三种季铵盐表面活性剂均对BSA内源荧光有猝灭作用,并导致其最大发射波长蓝移;表面活性剂的烷基链越长,Stern-Volmer猝灭常数和虚拟结合常数越大,表面活性剂与BSA的结合作用也越强.

英文摘要：

Interactions between a series of N-alkyl-N,N-di（2-hydroxyethyl）-N-methylammonium bromides with alkyl chain from dodecyl to cetyl and bovine serum albumin （BSA） in Tris-HCl buffer solution （pH=7.1） were studied by fluorescence spectroscopy at 298 K. Effects of the surfactant structure and BSA concentration on the interactions were examined. The interaction mechanism between BSA and surfactants at a relatively low concentration range was discussed using the Stern-Volmer equation and a model of the pseudo association constant. Each of the quaternary ammonium surfactants has a fluorescence quenching effect on BSA and leads to a blue shift of the maximum emission wavelength. The Stern-Volmer quenching constant and the pseudo association constant increase, which indicates that the interactions between surfactants and BSA become stronger, as the hydrophobic chain lengths increase.