中文摘要：

细胞极性对于细胞的多样性起着很重要的作用。发动蛋白是一个大的GTP酶,作用于胞吞作用和肌动蛋白的动力学过程。C.elegans中发动蛋白的同源基因dyn-1起着维持早期细胞极性的功能。我们对C.elegans中dyn-1基因进行了克隆,并构建到表达载体和RNAi载体中。经IPTG诱导表达得到了约90 kDa的DYN-1融合蛋白。同时,利用RNAi方法研究了dyn-1基因沉默后对三种线虫虫株N2、daf-2（e1370）和daf-16（e1038）寿命的影响。C.elegans在喂食dyn-1 RNAi食物后寿命明显缩短,也会导致严重的不育和胚胎致死。

英文摘要：

Cell polarity is crucial for the generation of cell diversity. Dynamin, a large GTPase, functions in both endocytosis and actin dynamics. Here, the C. elegans dynamin ortholog, DYN-1, maintains anterior polarity cues. dyn-1 was amplified from C. elegans cDNA by PCR and cloned into an expression vector and a RNA interference vector. The effects ofdyn-1 on lifespan of N2, dqf-2（e1370） and daf-16（e1038） were studied by RNAi. The lifespans of N2, daf-2（e1370） and daf-16（e1038） were shortened significantly. In this study, we also observed sterility and embryonic lethality in the dyn-1 RNAi-treated worms.