中文摘要：

Caenorhabditis elegans中的cep-1基因与哺乳动物中的肿瘤抑制因子p53同源，能够引发由DNA损伤导致的细胞凋亡。cep-1基因与寿命的关系研究并不多。我们利用C．elegans的cDNA进行PCR扩增得到cep-1的片段并构建到表达载体以及RNA干扰载体上，经过IPTG诱导表达得到了约90kDa的CEP-1融合蛋白。同时对C．elegans三种虫株：野生型，daf-2（e1370），daf-16（e1038）进行RNAi（gNA—mediated interference），发现cep—1 RNAi之后野生型的寿命显著延长，另外两种虫株的寿命没有显著改变，并以RT—PCR的方法检测了cep-1在这三种虫株中的表达情况。

英文摘要：

Caenorhabditis elegans cep-1 was similar to its mammalian counterpart, tumor suppressor p53. cep-1 was amplified from C.elegans cDNA by PCR and cloned into expression vector and RNA interference vector. The full length of cep-1 cDNA was 2106bp, containing a 5＇untranslated region of 27 bp and a 3＇untranslated region of 144 bp. The open reading frame was 1935bp which predicted encoding a 645 amino acids protein. CEP-1 recombinant protein was obtained by IPTG induced in Escherichia coli. At the same time, we investigated the effect ofp53/cep-1 on C.elegans lifespan in wild type, daf-2 （e1370）, daf-16 （e1038） strains by RNAi （RNA-mediated interference）, and found that the lifespan of wild type was prolonged significantly in cep-1 RNAi worm, in contrast, the lifespan of the other strains have no difference with the RNAi control worms. And the transcription level of cep- 1 in N2, daf-2 （e1370） and daf-16 （e1038） was determined by RT-PCR.