中文摘要：

目的 观察复合骨即重组人骨形态发生蛋白-2（rhBMP-2）/异体骨不同时间点融合骨组织中BMP-2、血管内皮生长因子（VEGF）的表达.方法 将新西兰大白兔60只随机分为3组,在L5、L6横突间行后路植骨融合术,分别植入复合骨条、自体骨条及异体骨条,于术后第1、2、3、4、5周取融合标本,用实时荧光定量逆转录聚合酶链反应（real time RT-PCR）分析内源性BMP-2和VEGF基因水平的变化.结果 术后第3周,复合骨组BMP-2为（5.3519±1.0384）,VEGF为（0.9257±0.2534）,均达到峰值且高于异体骨组和自体骨组（P〈0.05）,之后则缓慢下降.第4周后,内源性BMP-2表达仍保持较高水平,但VEGF的水平与自体骨组和异体骨组差异无统计学意义（P〉0.05）.结论 复合骨能有效地诱导内源性BMP-2和VEGF的表达,促进了成骨效应.

英文摘要：

Objective To investigate the endogenous bone morphogenetic protein （BMP）-2 and vascular endothelial growth factor （VEGF） gene expression of the masses taken from the rabbits undergoing posterolateral lumbar spinal fusion with rhBMP-2/allograft composite. Methods Sixty New Zealand white rabbits were randomly divided into three groups. The rabbits were subjected to L5, L6 intertransverse process fusion using rhBMP-2/allograft composite, autogenous iliac crest bone graft and allograft. Then the BMP-2 and VEGF expression was detected at the transcriptional level by real-time RT-PCR. Results At the 3rd week, the endogenous BMP-2 （5.3519±1.0384） and VEGF （0.9257±0.2534） gene transcription of the composite group reached the highest level, which was higher in comparison to autograft and allograft groups （P〈0.05）, and then declined. After 4 weeks, the expression of endogenous BMP-2 was still high, but VEGF level had no statistically significant compared to the other two groups （P〉0.05）. Conclusion The rhBMP-2/allograft composite could boost endogenous BMP-2 and VEGF gene expression effectively and improve the bone formation.