中文摘要：

目的 探讨CDC25在胰腺导管腺癌（PDAC）中的表达及意义。方法 分别收集8例手术切除的人胰腺癌、慢性胰腺炎、正常胰腺以及肝和（／或）淋巴结转移组织，采用Affymetrix公司的GeneChip HG-U95Av2 eDNA芯片初步筛查CDC25家族三个成员（CDC25A、B和C）在这些组织中的表达情况，然后通过定量PCR方法对基因芯片结果进行再验证分析。结果 基因芯片结果显示与慢性胰腺炎或正常胰腺组织比较，胰腺癌组织中CDC25B／mRNA的表达水平分别增加了1．4倍和1．9倍，而CDC25A和CDC25C mRNA水平在胰腺癌、慢性胰腺炎和正常胰腺组织中差异无统计学意义；定量PCR检测结果表明在胰腺癌组织中CDC25B／mRNA的平均表达水平分别比正常胰腺和慢性胰腺炎高7．5倍和3．9倍。结论CDC25B在胰腺癌细胞周期调控中起着重要作用，可能参与了胰腺癌的发生、发展和演进过程，CDC25B有可能成为胰腺癌早期诊断和治疗的一个靶点。

英文摘要：

Objective To investigate the expression of CDC25s in pancreatic ductal adenocarci- noma （PDAC） in order to elucidate the role of these genes as modulators of cell cycle progression in this disorder. Methods CDC25 mRNA expression levels in 32 pieces of tissue samples,which consisted of 8 cases of human pancreatic cancer,8 cases of chronic pancteatitis,8 cases of normal pancreas, as well as 8 cases of liver and（or） lymph node metastatic tissues were analyzed by cDNA array. Quantitative PCR was carried out to validate miccroarray results. Results DNA arrays analysis revealed that CDC25B but not CDC25A or CDC25C was significantly overexpressed in pancreatic cancer tissues compared to either chronic pancreatitis or normal pancreatic tissues. In contrast, there was no significant change in CDC25A and CDC25C mRNA levels among pancreatic dancer, chronic pancreatitis and normal pancreatic samples. Quantitative PCR showed CDC25B mRNA was also overexpressed in pancreatic cancer as higher as 7.5 times and 3.9 times in comparison to the normal pancreas and chronic pancreatitis respectively. Conclusion Our findings demonstrate an important role of CDC25B in cell cycle progression of pancreatic cancer cells,raising the possibility that CDC25B may have an early diagnostic and therapeutic potential in this disorder.