为探讨大鼠体内重要组织器官红桂木凝集素(Artocarpus lingnanensis lectin,ALL)受体的表达情况,本研究利用亲和层析法将红桂木凝集素进行分离纯化,并标记上辣根过氧化物酶,最后将大鼠各重要组织器官的石蜡切片进行凝集素组化分析。结果发现大鼠各重要组织器官都分布有不同程度ALL受体。在肺、心、脾和肾组织,ALL受体阳性率分别为:63%、54%、29%和24%。ALL受体主要分布在肺泡上皮细胞、心肌微血管内皮细胞、脾脏红髓区和肾脏的肾小球内皮细胞。
To explore the expression of Artocarpus Lingnanensis Lectin(ALL) receptor in some important tissues of rats,in the present study,Artocarpus Lingnanensis Lectin was first purified from the seeds of Artocarpus Lingnanensis by using affinity chromatography,labeled with horseradish peroxidase. Lectin-histochemistry was used to detect the ALL receptor of rats in some important tissues. Different expression level of ALL receptor was observed in most tissue,and the ALL receptor positive cells were 63%,54%,29% and 24% in lung,heart,spleen and kidney,respectively. The ALL receptor mainly located in alveolar epithelial cells,cardiac microvascular endothelial cells,spleen red-pulp region,and renal glomerular endothelial cells.