中文摘要：

本研究通过将临床分离的金黄色葡萄球菌（金葡菌）834菌株以不同的感染剂量经乳腺基部直接注射和剪断乳头尖端后经乳导管注射2种途径感染泌乳母鼠，建立了乳腺炎病理模型，并对感染后母鼠乳腺眼观病理变化、乳腺组织的病理学变化和乳腺中金葡菌数进行了比较研究。结果表明，与乳腺基部直接注射相比，剪断乳头尖端后经乳导管注射法可诱发更为典型的乳腺感染，不仅乳腺中感染菌数多、病理变化明显，而且试验结果的重复再现性好，是一种良好的小动物金葡菌乳腺感染模型。该模型的建立，将为进一步研究金葡菌乳腺炎的病原特性、发病机理、治疗方法和疫苗开发，提供可靠的小动物模型。

英文摘要：

Mastitis models were developed by inoculating mammary gland of lactating mice with different doses of Staphylococcus aureus isolate 834 through the route of injecting directly into the base of mammary gland or into the teat canal after cutting the very near end of teats. The clinical signs, histopathology, and bacterial growth in the mammary gland of the two models were compared. The results indicated that injecting into the teat canal after cutting the very near end of teats induced more typical mammary infection for the higher number of bacteria in mammary, more distinctive histopathology and better reproducible. This model could be useful for research of pathogenesis, treatment method and vaccine evaluation of Staphylococcus aureus-induced mastitis.