中文摘要：

目的观察不同浓度、不同时间P物质(substance P,SP)对骨髓间充质干细胞源性成骨细胞矿化的影响,并用SP非特异性的阻滞剂和NK1受体特异性的阻滞剂进行干预,从而推测其作用细胞的靶受体。方法 10只雄性6周龄SD大鼠制作脊髓损伤模型。从模型的胫骨、股骨提取骨髓间充质干细胞(mesenchymal stemcells,MSCs)进行成骨细胞的诱导培养,用第二代细胞进行实验。施加不同浓度的SP,对照组不加任何处理因素,在不同的时间点观察骨髓MSCs源性成骨细胞的矿化。并用SP、Spantide(SP的抑制剂)、L703606(NK1受体的抑制剂)进行配伍作用,观察骨髓MSCs源性成骨细胞的矿化。结果不同浓度的SP对细胞的矿化有不同程度的抑制作用,10~(-8)M浓度的抑制作用最强,矿化结节面积较对照组平均减少了38.3%(P<0.01),随时间的延长抑制作用越强(3W:-34.9%,P<0.01)。细胞矿化的抑制可以被Spantide和L703606所逆转,与SP组比较两组矿化分别升高了45.3%和50.5%。结论 SP通过NK1受体对成骨细胞的矿化产生影响。SP抑制了成骨细胞的矿化,使成熟的成骨细胞减少...

英文摘要：

Objective To investigate the mineralization of osteoblasts after treated with SP in different density and different time course.Nonspecific and specific receptor blocking agents of SP were used to identify target receptors mediated SP action.Methods MSCs from tibiae and femurs in SCI rats were cultured under control mediums and induced to become osteoblasts.Cell mineralization was measured by alizarin red staining with different experimental conditions including various density,time,and blocking agents.Resu...