中文摘要：

目的：利用纳米金颗粒提高复杂体系基因组低拷贝基因PCR扩增的反应特异性。方法：首先，模拟复杂基因组扩增模式体系，以接近单拷贝的hDNA为模板，在PCR过程中加入纳米金颗粒，设计优化实验，以便模拟建立复杂基因组低拷贝目的基因PCR扩增的模式体系。随后，扩增人类基因组的疾病相关的低拷贝基因模板（如人基因组肿瘤坏死因子基因外显子1的380bp），以检验纳米金优化增强PCR反应特异性的实际效果。结果：在复杂体系基因组的低拷贝基因PCR扩增中，纳米金颗粒能够较好地增强其PCR反应的特异性。结论：初步表明基于纳米金的纳米粒子PCR方法可以对复杂的实际基因组体系低拷贝基因的PCR扩增起到优化作用，这对于PCR反应优化方法的改进、推广具有重要的参考价值。

英文摘要：

Objective：Addition of gold nanoparticles （average diameterl0nm） into amplification system with low copy gene fragments from the complicated genome helps to enhance the specificity of PCR amplification. Methods：PCR amplification model system of the complicated genome was simulated, PCR was performed with approximate single copy k DNA as the template and addition of gold nanoparticles. The experiments were so repeated and optimized that the PCR amplification model system was built by simulating the PCR amplification of the complicated genome with low copy gene. Then, the low copy gene （tumor necrosis factor, TNF-ct gene exon 1 with 380bp ） of human genome was amplified so as to verify the actual efficiency of enhancement of the specificity of PCR amplification by the gold nanoparticles. Results：The gold nanoparticles could better enhance the specificity of PCR amplification in the PCR amplification of the complicated genome system with low copy gene. Conclusion：It was preliminarily indicated that nanoparticle PCR method based on gold nanoparticles could optimize the PCR amplification of the complicated actual genome system with low copy gene, which made an important valuable reference to improvement and extension of the optimized methods of PCR amplification.