中文摘要：

人工的介绍抗原的房间被期望在注入前刺激 T 房间的最佳的治疗学的特征的扩大和获得。这里，绑在 IgG monoclonal 抗体的可结晶的碎片的 CD32 遗传上在人的 K562 白血病房间上被表示为 T 房间受体提供 ligand。CD86 和 4-1BBL，它是分别地， CD28 和 4-1BB 的受体是的共同刺激的 ligands 也在 K562 上表示了房间。然后，我们由对 CD3 与 OKT3 monoclonal 抗体联合 K32/CD86/4-1BBL 房间完成了人工的介绍抗原的房间，命名 K32/CD86/4-1BBL/OKT3 房间。这些人工的修改细胞有导致 CD8+ T 细胞激活的能力，支持 CD8+ T 细胞增长，分割，和长期的生长，禁止 CD8+ T 细胞 apoptosis，并且提高 IFN- 和 perforin 的 CD8+ T 细胞分泌物。而且，抗原特定的细胞毒素的 T 淋巴细胞能在至少在 28 天以内与 K32/CD86/4-1BBL/OKT3 房间刺激的文化被保留。这条途径为 CD8+ T 房间的扩大和激活柔韧、简单、可再现、节俭并且可以为采纳免疫疗法有重要治疗学的含意。

英文摘要：

Artificial antigen-presenting cells are expected to stimulate the expansion and acquisition of optimal therapeutic features of T cells before infusion. Here CD32 that binds to a crystallizable fragment of IgG monoclonal antibody was genetically expressed on human K562 leukemia cells to provide a ligand for T-cell receptor. CD86 and 4-1BBL, which are ligands of co-stimulating receptors of CD28 and 4-1BB, respectively, were also expressed on K562 cells. Then we accomplished the artificial antigen-presenting cells by coupling K32/CD86/4-1BBL cell with OKT3 monoclonal antibody against CD3, named K32/CD86/4-1BBL/OKT3 cells. These artificial modified cells had the abilities of inducing CD8^＋ T cell activation, promoting CD8^＋ T cell proliferation, division, and long-term growth, inhibiting CD8^＋ T cell apoptosis, and enhancing CD8^＋ T cell secretion of IFN-T and perforin. Furthermore, antigen-specific cytotoxic T lymphocytes could be retained in the culture stimulated with K32/CD86/4-1BBL/OKT3 cells at least within 28 days. This approach was robust, simple, reproducible and economical for expansion and activation of CD8^＋ T cells and may have important therapeutic implications for adoptive immunotherapy. Cellular ＆ Molecular Immunology.