中文摘要：

目的 观察多器官功能障碍综合征（MODS）大鼠小肠胆碱能／氮能神经-Cajal间质细胞（ICC）网络形态学的变化并探讨其意义。方法Wistar大鼠随机分为对照组（20只）、MODS模型组（20只），行小肠肌层全厚标本c—Kit和乙酰胆碱囊泡状转运体（VAChT）／神经性一氧化氮合酶（nNOS）免疫荧光双重标记，共聚焦显微镜观测小肠神经-ICC网络结构的变化并进行定量分析。结果对照组单个高倍视野ICC和胆碱能／氮能神经纤维数量分别为：（18．0±2．2）个，（28．0±1．3）个，（16．0±0．3）个；c—Kit和VAChT／nNOS免疫阳性荧光强度分别为：（30．0±3．4）、（208．0±10．4）、（222．0±4．4）U；MODS模型组ICC和胆碱能／氮能神经纤维数量分别为：（6．0±0．5）个，（13．0±1．1）个，（7．0±0．5）个；c—Kit和VAChT／nNOS免疫阳性荧光强度分别为：（8．0±1．2）、（98．0±9．1）、（96．0±7．9）U；对照组各组数据与MODS模型组相应的数据比较有明显的差异（P〈0．01），MODS组小肠神经-ICC网络结构不连续。结论 MODS大鼠小肠胆碱能／氮能神经-ICC网络结构受到明显破坏，MODS大鼠胃肠运动障碍可能与小肠神经-ICC网络损伤有关。

英文摘要：

Objective To observe the damage of enteric cholinergic/nitrergic nerve-interstitial cells of Cajal （ICC） network in rats with multiple organ dysfunction syndrome （MODS）. Methods Forty Wistar rats were randomly divided into control group （ n = 20 ） and MODS model group （ n = 20 ）. The cholinergic/nitrergic nerve-ICC network in the intestine was observed using c-Kit and VAChT/nNOS immunohistochemical double-staining with whole-mount preparation technique and confocal laser scanning microscopy. Results In control group, the number of c-Kit-positive ICCs, VAChT/nNOS-positive fibers in one optical section was （ 18.0 ±2.2）, （28.0 ± 1.3） and （ 16.0 ±0.3）, respectively, and the intensity of immunoreactivity was （30.0 ± 3.4）, （208.0 ± 10.4） and （222.0± 4.4） U, respectively. In the MODS group, the number of c-Kit-positive ICCs, VAChT/nNOS-positive fibers in one optical section was （ 6.0 ± 0.5 ）, （ 13.0 ±1.1 ） and （7.0 ± 0.5 ）, respectively, and the intensity of immunoreactivity was （8.0 ±1.2）, （98.0±9.1） and （96.0 ±7.9） U, respectively. Compared with those in control group, the distributions and densities of cholinergic/nitrergic nerves and ICC in the intestine of model group were significantly decreased （P 〈0.01 ）, and the enteric nerve-ICC network was discontinued. Conclusion The enteric nerve-ICC network was disrupted in MODS, which may be associated with the disorder of gastrointestinal motility.