中文摘要：

目的：探讨乳腺癌组织中C—erbB2的表达与患者外周血微转移的相互关系及临床意义。方法：用巢式逆转录-聚合酶反应（Nested—liT—PCR）检测60例乳腺癌患者术前外周血SBEM—mRNA的表达，对其术后切除的组织标本用免疫组化S—P法检测c—erbB2的表达情况。结果：60例乳腺癌中，C—erbB2阳性表达27例，在相应外周血中SBEM—mRNA的阳性表达率为59．3％（16／27），在C—erbB2阴性表达组，SBEM—mR—NA阳性率为24．2％（8／33）。二者差异有统计学意义（P〈0．01）。结论：乳腺癌组织中C—erbB2的高表达与外周血微转移显著相关，C—erbB2在判断乳腺癌患者不良预后上有重要的临床意义。

英文摘要：

Objective：To explore the expression of c - erbB2 and its clinical significance as well as the relationship with micrometastases in peripheral blood in breast cancer. Methods： SBEM - mRNA was detected by reverse transcriptase -polymerase chain reaction（RT- PCR） in blood as an index of micrometastases in 60 breast cancer patients before operation. The expression of c - erbB2 in breast cancer was examined by immunohistochemistry method（S - P） after operation. Results： In 60 breast cancer patients, the positive cases of c - erbB2 were 27. The posi- tive rate of SBEM - mRNA in peripheral blood with c - erbB2 positive disease and negative disease were 59.3% （16/27） and 24.2% （8/33）, respectively. There was a significant difference between the two groups（ P 〈 0.01 ）. Conclusion： High expression of c - erbB2 is closely related with the micrometastases in peripheral blood in breast cancer, and reflect an enormous clinical significance by inferring unfavourable prognosis in breast cancer