中文摘要：

目的：通过检测人类原发性乳腺癌患者外周血中乳腺小粘蛋白（SBEM）mRNA和癌胚抗原（CEA）的表达情况，同时结合患者其他相关临床病理学资料，探讨二者检测在乳腺癌患者中的临床意义。方法：采用逆转录-聚合酶反应（RT—PCR）和酶联免疫反应（ELESA）方法分别检测60例乳腺癌患者外周血中SBEM—mRNA和CEA的表达情况，另抽取20例乳腺纤维腺瘤患者和10例健康人的外周血标本加以检测，作为对照研究纽。采用SPSS 10．0软件进行统计学处理。结果：在60例乳腺癌患者外周血中SBEM—mRNA和CEA的阳性表达率分别为53-3％和31．7％。而在对照组检测中，CEA在纤维腺瘤患者外周血中阳性率为5．0％，在健康人的外周血中为阴性表达；SBEM—mRNA在二者中均为阴性表达。统计学分析发现，乳腺癌患者中SBEM—mRNA和CEA的阳性表达均与腋淋巴结转移情况、肿瘤TNM分期差异有显著性意义（P〈0．05），而与患者的其他临床病理学特征的差异无统计学意义（P〉0．05）。对比研究表明，二者联合检测比单项检测有更高的敏感度和准确性（Pl〈0．05）。结论：乳腺癌患者外周血中SBEM—mRNA和CEA的检测对指导乳腺癌患者的治疗和判断预后具有重要意义，二者联合检测在提高对乳腺癌微转移敏感度和准确性的同时，又保持了较高的特异性，是较为理想的检测组合方式。

英文摘要：

Objective： To detect the expression of small breast epithelial mucin （SBEM） mRNA and carcinoembryonic antigen （CEA） glycoprotein in primary breast cancer and to investigate their clinical significance. Methods： The levels of SBEM mRNA and CEA glycoprotein were detected by RT-PCR and ELISA in the peripheral blood of 60 patients with breast cancer, 20 patients with fibroadenoma and 10 healthy adults. We used SPSS10.0 to analyze the experimental data. Resultsi The positive rates of SBEM mRNA and CEA glycoprotein expression in the peripheral blood of patients with primary breast cancer were 53.3% and 31.7%, respectively. In the peripheral blood of the 20 patients with fibroadenoma, SBEM mRNA expression was 0% and CEA glycoprotein expression was 5.0%. In the peripheral blood of the 10 healthy controls, the expression of SBEM mRNA and CEA glycoprotein was 0%. The expression of SBEM mRNA and CEA glycoprotein was correlated with axillary lymph node metastasis and TNM stage （P〈0.05） but was not correlated with other clinical characteristics （P〉0.05）. A combined test to detect serum SBEM mRNA and CEA glycoprotein showed increased accuracy and sensitivity （P〈0.05）. Conclusion： Combined detection of SBEM mRNA and CEA glycoprotein is valuable for the treatment and prognosis of patients with primary breast cancer.