中文摘要：

目的：建立大鼠血浆中延胡索乙素浓度的高效液相色谱-荧光检测法。方法：色谱柱为Phenomenex C18色谱柱（250mm×4．6mm，5μm）；流动相为乙腈-0．1％磷酸（用三乙胺调至pH6．15）（60：40）；柱温：30℃；流速：1．0mL·min^-1；荧光检测器：激发波长：231nm，发射波长：324nm：进样量：60μL.结果：标准曲线的线性范围为5～1000ng·mL^-1（r=0．9989，n=8），低、中、高3种浓度的绝对回收率分别为（90．1±2．4）％、（94．1±2．7）％、（93．6±3．0）％；方法回收率分别为（93．4±1．2）％、（96．1±2．7）％、（95．8±1．5）％；日内精密度RSD≤2．3％，日间精密度RSD≤4．0％。本方法的检测限0．5ng·mL^-1。结论：本方法快速、简便、灵敏、准确.

英文摘要：

Objective： To establish a method for determination of tetrahydropalmatine in rats plasma by RP-HPLC-FD. Methods： Chromatographic conditions included an Phenomenex C18 column （4.6 mm × 250 mm, 5 μm）, a mobile phase of a mixture of acetonitrile -0.1% H3PO4 （adjusted to pH 6.15 with triethylamine） - （60： 40） with a flow rate of 1.0 ml·min^-1, a fluorescence detector with excitation wavelength at 231 nm and emission wavelength at 324 nm. Results： The linear ranges of the plasma samples were 5 - 1000 ng· mL^-1 （ r = 0. 998 9, n = 8 ）. Within the range, the absolute recoveries of three different concentrations were （90.1 ± 2.4） %, （94.1 ±2.7 ） %, （93.6 ± 3.0） % , analytical recoveries were （93.4 ±1.2） % , （96.1 ±2.7 ） % , （ 95.8 ±1.5 ） % , and the within-day RSD and between-day RSD were less than 2.3% and 4.0%. The detection limit was 0.5 ng· mL^-1. Conclusions：The method was simple quick and sensitive.