中文摘要：

目的探讨视网膜神经胶质细胞对小鼠骨髓血管内皮前体细胞（EPCs）迁移的作用及可能机制。方法荧光免疫激活流式细胞仪分选获得小鼠骨髓血管内皮前体细胞,分别用神经胶质细胞或对照组成纤维细胞作为条件共培养细胞与EPCs细胞共培养24h后,检测内皮前体细胞通过孔隙的迁移数量;并用ELISA法检测培养液内VEGF及SDF-1的含量。结果 CD34/VEGFR2双标阳性的细胞为富含骨髓血管内皮前体细胞的细胞群,约占全部骨髓细胞的0.2%;当EPCs神经胶质细胞共培养24h后,EPCs通过孔隙的数量显著高于对照组与成纤维细胞共培养时通过的数量（上层存留细胞（20±16）个v（s170±55）个,P〈0.01）。与神经胶质细胞共培养系统细胞培养液中VEGF及SDF-1含量显著高于对照组[VEGF：（230.28±27.37）pg/mL vs（89.22±11.23）pg/mL,P〈0.01;SDF-1：（328.34±57.42）pg/mL vs（62.44±34.35）pg/mL,P〈0.01]。结论神经胶质细胞可能通过细胞因子的分泌作用增强EPCs的移行,在EPCs参与的新生血管形成中可能有重要的介导作用。

英文摘要：

【Objective】 To evaluate the migration of bone marrow derived EPCs mediated by retinal glia in an experimental transwell model as well as the level of the related cytokines released by glia.【Methods】 Primary culture retinal cells were incubated in the lower chamber of the transwell system;enriched EPCs（CD34/VEGFR2 double-positive cells） isolated by FACS from the mouse bone marrow were harvested and seeded on the upper chamber.Effects of co-culture with retinal glia cells on the migration of EPCs were evaluated in a transwell experimental model with a 5μm hole as compared with the control fibroblast cells.The level of cytokine VEGF and SDF-1 in the medium of transwell were detected by ELISA.【Results】 Co-culture with retinal glia cells lead to a significant increase of transmigration of EPCs from the upper chamber to the lower chamber as compared with the control fibroblast cells.The VEGF and SDF-1 released to the media in the glia group were much higher than the control group [VEGF：（230.28±27.37）pg/mL vs（89.22±11.23）pg/mL vs P 0.01;SDF-1（328.34±57.42）pg/mL vs（62.44±34.35）pg/mL,P 0.01].【Conclusions】 Co-culture with glia cells from retinal cells was capable of activating migration of bone marrow derived EPCs in an experimental transwell model.Analysis of the effects of retinal glia cells in the transwell on EPC＇s migration might be useful in providing effective experimental means for the identification of the specific signaling molecules involved in the activation of EPCs migration for the regeneration of retinal blood vessels.