高山松是云南松和油松自然杂交产生的二倍体杂种,不同居群形成的遗传背景及生境差异很大.本研究以来自该物种分布区内有代表性的8个居群为试材,在理论亲本种之一即云南松生境下比较其萌发特征.结果表明:不同居群种子萌发动态表现出明显的"S"型曲线,拟合模型检验均达极显著水平,应用Logistics方程拟合效果较好,整个萌发期可分为萌发初期、萌发快速期和萌发后期;不同居群在萌发物候和萌发数量上存在一定的差异,萌发快速期起始时间为7~9 d,结束时间为11~15 d,而快速期持续时间较稳定;不同居群萌发率为52.12%~88.94%,平均73.65%,其中萌发快速期的萌发率为33.10%~49.94%,平均42.28%,萌发快速期占整个萌发期比率较大,分别为47.14%~66.40%,平均为58.20%;居群间的分化表现为以分布区东北部紧接油松分布的MK居群萌发最早,速度最快,萌发率最高,LX次之,而NY居群萌发率最低,萌发速度最慢,其它居群介于中间.快速期萌发率与总萌发率间存在显著正相关关系,相关系数为0.772 5,拟合值与实测值呈极显著的相关关系,相关系数为0.999 5.这些结果表明,高山松在理论亲本种之一即云南松生境下也表现出正常的萌发能力,且不同居群间存在明显的分化,这与高山松的杂交起源特性及不同居群复杂的遗传背景有关.
Pinus densata originated through natural hybridization between P.yunnanensis and P.tabulaeformis.The individual hybrid origin nature of P.densata among populations has been borne out.In present study,seeds harvested from eight representative populations distributed throughout its natural rang were germinated aiming to explore germination characteristics and compare population divergence under natural field conditions environment in putative parental species P.yunnanensis habitat.The results showed that the dynamic regularity of the germination percentage can be fitted by an "S-shaped" curve as "slow-fast-slow" and can be simulated with Logistic equations.The germination period was divided into 3 stages according to its germination percentage: initiation stage of seed germination,rapid stage of seed germination and later stage of seed germination.There were differences in germination time and germination number among populations.The beginning and end of the rapid stages for seed germination were 7—9 days and 11—15 days respectively.The duration of the rapid germination stages were relatively stable.The germination percentages were 33.10 %~49.94 % and 52.12 %~88.94 % in the rapid stage and in the whole period among populations,respectively.The number of the seed germination in the rapid stage had high ratio compared to the whole period,and the ratios were 47.14 %~66.40 %.MK population located in the northeastern periphery of the P.densata range adjacent to the P.tabuliformis had early seedling emergence and high germination percentage followed by LX population.By contrast,the NY population had late seedling emergence and low germination percentage.According to regression analysis,germination percentage in rapid stage and in whole period was liner correlation,and the correlation coefficient was 0.7725.Extremely significant correlations were found between the smiulated values and the actual measured values of seed germination percentage,and the correlation coefficient was 0.9995.All results suggested th