中文摘要：

利用紫外线-60 Co-γ、微波及硫酸二乙酯诱变新月弯孢霉菌株，使其产漆酶能力大幅度提高。漆酶活性由原来的0．55μmol/（ min·mL）提高到1．949μmol/（ min·mL）。进一步通过单因素实验及正交实验得出最佳产酶条件为：发酵温度30℃，发酵时间72 h，培养基pH为6，装液量120 mL，摇床转速160 r/min，土豆200 g/L，蔗糖30 g/L，大豆蛋白胨5 g/L， KH2 PO45 g/L，愈创木酚0．2 mmol/L，吐温800．1 mL/L， Mg2＋0．5 mmol/L， Cu2＋1 mmol/L，Ca2＋1 mmol/L，Mn2＋0．25 mmol/L。在此条件下，最终漆酶活性可达2．306μmol/（ min·mL）。

英文摘要：

In this paper,Curvularia lunata strains were mutagenized by UV-60 Co-γ,microwave and diethyl sulfate. By means of the mutation treatment, the laccase ability of C. lunata substantially increased from 0.55 μmol/（ min·mL） to 1.949μmol/（ min·mL） . In order to increase the laccase ability further, the single factor experiment and orthogonal de-sign experiment were carried out, the result indicated that the optimal conditions were as follows：fermentation tempera-ture 30 ℃, fermentation time 72 h, culture medium pH 6, liquid volume 120 mL, rotation rate 160 r/min, potato 200 g/L, sucrose 30 g/L, soy peptone 5 g/L, KH2 PO4 5 g/L, guaiacol 0.2 mmol/L, Tween 80 0.1 mL/L, Mg2＋0.5 mmol/L, Cu2＋1 mmol/L, Ca2＋1 mmol/L, Mn2＋0.25 mmol/L. Ultimate laccase activity 2.306 μmol/（ min·mL） .