中文摘要：

选用50头体重相近的三元杂交断奶健康仔猪，随机分为5组：对照组全程饲喂玉米-豆粕型基础日粮；试验1、2、3、4组在试验第1天到第28天分别饲喂基础日粮＋蜡样芽孢杆菌、鼠李糖乳杆菌和戊糖乳杆菌（质量比为1：1：1、1：2：1、2：1：2、1：1：2）的混合益生菌制剂，在第29天到第42天饲喂基础日粮。饲喂28d后计算各组仔猪13增重、料重比、腹泻率。结果表明：4个试验组仔猪的13增重均高于对照组，料重比均低于对照组，腹泻率均显著低于对照组；试验1、3组仔猪的日增重显著高于对照组，且试验1组仔猪的日增重又稍高于试验3组；试验1、3组仔猪的料重比显著低于对照组，而试验1组仔猪的料重比又稍低于试验3组；试验1组和3组仔猪的腹泻率相同，且低于试验2组和4组，表明4种比例混合的益生菌制剂均能促进仔猪生长，且以质量比1：1：1、2：1：2混合的益生菌制剂促进仔猪生长的效果较好；对试验1组和对照组仔猪在试验0、7、14、21、28、42d肠道内菌群的定量PCR结果表明，试验1组仔猪肠道内蜡样芽孢杆菌、鼠李糖乳杆菌数量在饲喂益生菌制剂期间呈现一致的变化趋势，即先上升后下降，再上升，而对照组3种菌的变化趋势不一致；在停喂益生菌制剂2周后，3种益生菌仍呈规律变化，表明饲喂益生菌制剂能够调节仔猪肠道相应菌群的生长；对仔猪肠道粪样菌群的16SrDNA的特异序列的进行变性梯度凝胶电泳及抠胶测序的结果表明，仔猪在饲喂益生菌制剂7d后，肠道蜡样芽孢杆菌、肠球菌、链球菌、双歧杆菌、戊糖乳杆菌、大肠杆菌、鼠李糖乳杆菌菌群数量均下降，到第28天时，各种菌群数量均上升，表明饲喂益生菌制剂不仅能调节仔猪肠道相应益生菌的生长，还能调节肠道肠球菌、链球菌、大肠杆菌与双歧杆菌的生长。

英文摘要：

Fifty weaned healthy piglets with similar weight were randomly assigned to five groups： control group fed with corn-soybean meal diets during all the process; test group 1, 2, 4 and 4 first fed with basal diet added with probiotics containing Bacillus cereus, Lactobacillus rhamnosus, and Lactobacillus pentosus mixed in ratio of 1 ： 1 ： I, 1 ： 2 ： 1, 2 ： 1 ： 2 and 1 ： 1 ： 2, respectively from the 1st to the 28th day of the experiment, then with basal diet from the 29th day to the 42nd day. Daily gain, feed/weight ratio, diarrhea rate were calculated after 28 days of feeding. The results showed that daily gain was higher, feed/weight ratio was lower and diarrhea rate was significantly lower in piglets of test groups compared to the control. Daily gain of piglets in test group 1 and 3 was significantly higher than that in the control and in test group 1 was slightly higher than in group 3. Feed/weight ratio of piglets in test group 1 and 3 was significantly lower than that in the control and in test group 1 was slightly lower than in group 3. Piglets in test group l and 3 showed the same diarrhea rate which was lower compared to test group 2 and 4. These results indicate probiotics with mixing ratio of 1 ： 1 ： 1 and 2 ： 1 ： 2 have better growth promoting effect for piglets. Quantitative PCR was applied on samples of test group 1 and control group collected after 0, 7, 14, 21, 28, 42 d from the beginning of the experiment, the results showed Bacillus cereus, Lactobacillus rhamnosus, and Lactobacillus pentosus in intestine of piglets in test group 1 exhibited the same growth tendency during feeding the probiotics, which increased and then decreased and increased again, while the 3 strains in intestine of piglets of control group didn＇t show such same tendency. The 3 strains still showed regular changes 2 weeks after ceased feeding the probiotics, indicating probiotics could regulate the growth of the correspond bacteria in intestine of the piglets. Denaturing gradient gel electrophoresis ?