中文摘要：

为确定猪捷申病毒（PTV）Swine／CH／IMH／03株的易感细胞系及其致病性，本实验将该病毒接种不同细胞，通过细胞病变、电子显微镜观察和PCR技术进行鉴定。并进一步将该病毒回归本动物鉴定其致病性。试验结果表明，PTv swine／cH／IMH／03对IB．RS-2和PK-15细胞系敏感，能够在IB-RS-2和PK-15细胞系中增殖。致病性试验结果显示，Swine／cH／IMH／03分离株能够导致实验猪出现神经症状，剖检可见脑、肾、脾、肠道发生出血，病理组织学检查可见脑实质内胶质细胞浸润、肾脏间质小血管淤血、脾淋巴细胞减少、小肠伴有出血及淋巴细胞浸润等病理变化，对仔猪具有强致病性。

英文摘要：

To determine the permissive cell lines for porcine Teschovirus （PTV） isolate of Swine/CI-UIMH/03 and evaluate the pathogenicity of the virus, the virus was inoculated to difference cell lines for virus replication tests. The results showed virus was able to efficiently replicate in IB-RS-2 and PK-15, which were identified by cytopathic effect, electron microscopy observation in the cell lines and PCR amplification of the virus 5＇UTR. Furthermore, the pathogenicity test result indicated that the PTV Swine/CH/IMH/03 was highly pathogenic to piglets, which caused pathological lesions including brain hemorrhage, pneumorrhagia, splenorrhagia, and enterorrhagia, and the histopathology changes associated with glial cell infiltration in brain parenchyma, renal interstitial vascular congestion, interstitial pneumonia, reduced splenic lymphocytes, and small intestine hemorrhage and lymphocyte infiltration.