中文摘要：

以麻疯树根、茎、叶、花和果实为材料，使用DSN（duplex-specific nuclease）均一化技术与SMART（switching mechanism at 5’end of RNA transcript）建库技术相结合，构建了麻疯树全植株均一化全长cDNA文库．经检测库容为1．4×10。个克隆，插入片段平均长度大于1kb．随机挑取200个克隆，利用PCR方法测得文库重组率达95％，48．95％的EST（Expressed Sequence Tag）没有同源性，显示了这些ESTs中能够发现新功能基因的可能性．与GeneBank Nr数据库中已知功能基因有较高同源性的有97条ESTs，相关同源性的已知基因分别参与基础代谢与运输、细胞构成、能量转换、辅酶代谢与运输、信号传导、翻译机翻译后的修饰折叠等过程．这是第一次以麻疯树全植株为材料构建全长均一化cDNA文库．

英文摘要：

A normalized full-length cDNA library was established using the root, stein, leaf, flower and fruit of Jatropha curcas L. by DSN （duplex-specific uclease）-normalization method combined with SMART （switching mechanism at 5＇ end of RNA transcript） technique. This library reaches 1.4 × 106 in capacity; PCR results showed that the average size of inserts was larger than 1000 bp with a recombination rate of 95%. From sequenced 200 clones at random, 48. 95% showed no similarity to known genes, suggesting a potential for further novel gene discovery. Blasted in GeneBank Nr database, 97 ESTs had high homology to known genes, which participate in basic metabolism and the transportation, cell structure, energy conversion, coenzyme metabolism and transportation, signal transduction, translation and folding process as well as modification after translation. This is the first time that a normalized full-length cDNA library was established using the whole plant of Jatropha curcas L. as materials.