中文摘要：

目的：基于UPLC-代谢组学技术表征大黄结合有氧运动对血脂异常大鼠血脂的调节作用。方法：用高脂饲料喂饲大鼠4周，同时进行4周的大黄、有氧运动、大黄与运动结合干预。实验第1d和第2周末眼眶静脉丛取血，第4周后处死动物，采用基于UPLC-代谢组学证明不同治疗组的治疗效果。比较血清TC、TG、HDL-C、LDL-C、SOD、GSH-Px、CAT和MDA的变化。结果：代谢组学与生化指标的结果结合证明在4周时间，血脂异常模型被成功建立，不同的时间段产生不同的损伤程度且明确了血脂异常模型的时间进展轨迹，显示了不同治疗组的对血脂异常的影响。代谢组学显示不仅大黄结合有氧运动组对血脂异常的有一定的治疗作用，而且二者结合的治疗效果优于单一大黄组或有氧运动组。生化指标显示大黄和有氧运动均可显著降低大鼠血清TC、TG和LDL-C提高、HDL-C水平；大黄结合有氧运动组大鼠血清SOD、GSH-Px、CAT显著升高同时MDA的水平下降，脂质过氧化产物水平显著降低。结论：大黄结合有氧运动可治疗血脂异常大鼠血脂异常。代谢组学显示血脂异常模型已成功建立，进而可推广代谢组学技术可以作为一种推测药理模型制作程度及是否成功的新方法，代谢组学也作为药物或有氧运动改善疾病程度及效果的一个新方法。

英文摘要：

Objective： UPLC-based metabonomics study on therapeutic effect of Rhubarb and aerobic exercise in dyslipidemia rat. Methods ： The rats were raised by high fat diet for 4 weeks ; meanwhile these rats were treated with Rhubarb and aerobic exercise for 4 weeks. The blood samples were collected from retro-orbital puncture with ether and transferred into tubes on the first day and the second weekend. Then those rats were executed, and the model of dyslipidemia was then established and the model was verified by metabonomics method. The serum TC, TG, LDL-C, HDL-C, GSH-Px, SOD, CAT and MDA were determined. Results： According to metabonomie method and biochemical parameters, the hyperlipemia rat model was successfully established in 4 weeks. The re- sults showed the time progress of metabolic disorders in the different periods. The results showed that Rhubarb and aerobic exercise could decrease the concentration of serum LDL-C, TC and TG remarkably and raise the concen- tration of HDL-C. The activity of GSH-Px, SOD and CAT in Rhubarb in combination with aerobic exercise group is promoted and the level of MAD is decreased distinctly. Conclusion： Rhubarb in combination with aerobic exer- cise could treat the abnormity of serum lipid and has obviously anti-lipoperoxidation effect. The metabonomic re- sult showed the model of dyslipidemia was then established. The metabolomics technology can be used as a new method whether successful pharmacology model established or not. Metabolomics could also be a new method for studying on the therapeutic effect of aerobic exercise.