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中文摘要：
目的：建立高速逆流色谱分离纯化紫苏叶中迷迭香酸的方法。方法：采用高速逆流色谱分离纯化紫苏叶乙酸乙酯萃取部分中迷迭香酸，以石油醚-乙酸乙酯-甲醇-0．5％醋酸水溶液（2：5：2：5）为溶剂体系，上相为固定相，下相为流动相，流速2．0mL·min^-1，主机转速800r·min^-1，温度25℃。结果：每0．20g紫苏叶乙酸乙酯萃取物经1次纯化，纯度为98．6％的迷迭香酸的得率约13％。结论：高速逆流色谱可高效分离纯化紫苏叶中的迷迭香酸。
英文摘要：
Objective： To establish a method for separation and purification of rosmarinic acid from Perilla frutescens（ L. ） Britt. vat. acuta （ Thunb. ） Kudo form. discolor（ Makino ） Kudo （ abbr： PFAD） by high - speed counter - current chromatography（ HSCCC ）. Methods： The compound was separated and purified by HSCCC from the ethyl acetate part of the crude extract of PFAD, using petroleum ether - ethyl acetate - methanol - 0. 5 % acetic acid water -solution（2： 5： 2：5 ）as the two -phase solvent system. The upper phase was chosen as the stationary phase, and the lower phase was chosen as the mobile phase. The mobile phase was operated at a flow rate of 2. 0 mL ·min^-1, while the apparatus rotated at 800 r ·min^-1 and the temperature was set at 25 ℃. Results： The yield of rosmarinic acid with 98.6% purity was 13% from each 0.20 g ethyl acetate part of the crude extract of PFAD by once separation. Conclusion： HSCCC is a efficient method for separating, and purificating rosmarinic acid from PFAD.
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