中文摘要：

目的将从老鼠主动脉调查在 endothelial 房间和光滑的肌肉房间之间的主要文化，纯化和生物特征的差别。方法 Endothelial 房间用脉管的戒指坚持， collagenase 消化方法和一个改进脉管的戒指坚持方法被获得，当光滑的肌肉房间与老鼠主动脉的织物节被分开时。endothelial 房间的克隆被限制冲淡试金选择。两种房间类型用特定的房间免疫被识别荧光灯的标记，和阶段对比显微镜学被用来在单个房间和殖民地水平观察在 endothelial 房间和光滑的肌肉房间之间的词法不同。房间增长被数 kit-8 的房间决定。在 cryopreservation.Results Endothelial 房间被所有三个方法获得以后， endothelial 房间和光滑的肌肉房间之间的差别在胰岛素消化时间，附件时间和恢复被评估。改进脉管的戒指方法提供了最可再现的结果。房间处于好状况，并且高纯净。光滑的肌肉房间由组织碎片文化方法成功地是有教养的。单个 endothelial 房间的同种细胞的扩大被达到。二种房间类型表示了他们的各自的特定的标记，并且光滑的肌肉房间的增长的率超过了 endothelial 房间的。Endothelial 房间比光滑的肌肉房间对胰岛素消化更敏感。另外，他们比光滑的肌肉房间有一更短的坚持时间和更好的恢复追随者 cryopreservation。结论改进脉管的戒指方法为产出 endothelial 房间是最佳的。限制冲淡是为从老鼠主动脉净化主要 endothelial 房间的一个新奇、有效的方法。主要老鼠 endothelial 房间和脉管的光滑的肌肉房间文化展出了不同词法特征，增长率，坚持时间，危险性到在 cryopreservation 以后的胰岛素消化和恢复。我们的研究能是为在血容器的新生的进一步的申请的一个好基础。

英文摘要：

Objective： To investigate the differences of primary culture, purification and biological characteristics between endothelial cells and smooth muscle cells from rat aorta. Methods： Endothelial cells were obtained using the vascular ring adherence, collagenase digestion method and an improved vascular ring adherence method, while smooth muscle cells were separated from tissue sections of rat aorta. Clones of endothelial cells were selected by limiting dilution assay. Both cell types were identified using specific cell immunofluorescent markers, and phase contrast microscopy was used to observe the morphological disparity between endothelial cells and smooth muscle cells at the single cell and colony level. Cell proliferation was determined by the cell counting kit-8. Differences between endothelial cells and smooth muscle cells were evaluated in trypsin digestion time, attachment time and recovery after cryopreservation. Results： Endothelial cells were obtained by all three methods. The improved vascular ring method provided the most reproducible results. Cells were in good condition, and of high purity. Smooth muscle cells were cultured successfully by the tissue fragment culture method. Clonal expansion of single endothelial cells was attained. The two cell types expressed their respective specific markers, and the rate of proliferation of smooth muscle cells exceeded that of endothelial cells. Endothelial cells were more sensitive to trypsin digestion than smooth muscle cells. In addition, they had a shorter adherence time and better recovery following cryopreservation than smooth muscle cells. Conclusion： The improved vascular ring method was optimal for yielding endothelial cells. Limiting dilution is a novel and valid method for purifying primary endothelial cells from rat aorta. Primary rat endothelial cell and vascular smooth muscle cell cultures exhibited different morphological characteristics, proliferation rate, adherence time, susceptibility to trypsin digestion and recovery after cryopreserva