中文摘要：

目的探讨中介素（IMD）对大鼠肾脏缺m再灌注损伤（IRI）的影响，及其过程中一氧化氮合酶（NOS）的作用和机制。方法将健康雄性wistar大鼠分为4组：假手术组，行右肾切除术，1周后单纯分离左侧肾蒂及肾动脉，而不夹闭肾动脉；肾脏缺血再灌注（IR）组，行右肾切除术，1周后行左肾缺血再灌注手术；IMD基因转染组，右肾切除后左。肾行超声微泡介导的IMD-pCDNA3．1（＋）质粒转染术，饲养1周，再行左肾缺血冉灌注手术；空质粒转染组，右肾切除后左肾行超声微泡介导的pCDNA3．1（＋）质粒转染术，饲养1周，再行左肾缺血再灌注手术。大鼠于缺血再灌注术后24h处死，用免疫组织化学方法检测。肾组织IMD表达，取肾组织进行病理学观察，取血清测定尿素氮（BUN）和肌酐（Cr）浓度，检测肾组织中内皮型NOS（eNOS）、诱导型NOS（iNOS）以及神经型NOS（nNOS）mRNA及其蛋白的表达。结果假手术组大鼠肾组织中IMD位于肾小管及问质细胞胞浆内，其表达灰度值为66±35；肾脏IR组大鼠肾小管上皮细胞和问质中IMD表达灰度值为176±48，高于假手术组（P〈0．01）；IMD基因转染组肾组织中IMD表达灰度值为262±68，高于肾脏1R组（P〈0．01）；空质粒转染组IMD表达灰度值为180±51，和肾脏IR组间表达的差异无统计学意义（P〉0．05）。与肾脏IR组相比较，IMD基因转染组大鼠肾脏组织病理损伤程度较轻，血清BUN和Cr较低（P〈0．05），eNOSmRNA及eNOS表达升高（P〈0．05），iNOSmRNA及iNOS表达降低（P〈0．05），而两组问nNOSmRNA及nNOS表达的差异无统计学意义（P〉0．05）。结论中介素可能通过促进eNOs表达、抑制iNOS表达从而减轻大鼠肾脏IRI。

英文摘要：

Objective To observe the effects ot intermedin （IMD） on nitric oxide synthetase （NOS） in renal isehemia reperfusion （IR） rat models and the action mechanism. Methods A total of 24 rats were divided into four groups （n = 6 each）. Group I underwent right nephreetomy one week prior to the exposure of left renal peclieles, but did not receive any I/R. Group Ⅱ underwent right nephreetomy one week prior to left renal I/R surgery. Group III underwent right nephreetomy and left renal IMDpCDNA3. 1 （ ＋ ） transfection by ultrasound-mircobubbles and renal I/R surgeries were performed one week after gene transfection. Group Ⅳ was treated with the same way as group III except that empty control vector was transfected. All the animals were killed at the end of 24 h of reperfusion. The expression and site of IMD were determined by using immunohistochemistry. Serum levels of BUN and creatinine were determined. The kidney formaldehyde-fixed and paraffiwembedded sections were stained with HE and PAS by standard methods and then histological changes were analyzed semiquantitatively. The mRNA expression levels of endothelial NOS （eNOS）, inducible NOS （iNOS） and neuronal NOS （nNOS） in the kidneys of the four groups were detected by using RT-PCR. The protein expression levels of the three NOS mentioned above in the kidneys were semiquantitatively analyzed by Western blotting. Results IMD was weakly expressed in the plasma of tubulointerstitial cells in sham operated group; whereas IMD expression in the kidneys subject to I/R was increased. Moreover, as compared with I/R group, IMD expression levels were obviously increased （P〈0. 01 ）. The degree of morphological changes as well as renal dysfunction in group Ⅲ was obviously lessened as comtmred with 12rouD Ⅱ. The mRNA and urotein exoression levels of eNOS in aroun Ⅲ werenotably increased as compared with group Ⅱ, while the mRNA and protein expression levels of iNOS in group Ⅲ were obviously reduced as compared with I/R group not tran