中文摘要：

目的：探讨EBVBARF1基因对人支气管上皮细胞生长特性的影响，并初步探讨其作用机制。方法：构建真核重组表达载体pcDNA3-BARF1，转染人支气管上皮细胞（HBEC），检测BARF1基因的表达，观察细胞生长状况、生长速度，在软琼脂中集落形成能力及对裸鼠的致瘤能力。结果：EBV—BARF1基因转染细胞生长旺盛，失去接触抑制能力，生长速度增快，在软琼脂中形成多个集落，并在裸鼠体内成瘤。结论：EBV的早期基因BARF1能明显地改变上皮细胞的生物学行为，使细胞发生恶性转化、获得肿瘤细胞的生长特性，该基因不仅与上皮细胞的早期永生化有关，在细胞的恶性转化及肿瘤形成过程中亦可能起重要的作用。

英文摘要：

Objective： To explore the effect of BARF1 gene of EB virus on growth features of human bronchial epithelial cells （HBEC） and the corresponding mechanism. Methods： pcDNA3-BARF1 was constructed with the eukaryotic recombinant expressing vector. HBEC were transfected with pcDNA3 or pcDNA-BARF1. The expression of BARF1 was detected. Growth dynamics of cultured ceils, colonic formation in soft agar, and the tumorigenicity of the transfected ceils in nude mice were observed. Results：The BARF1 gene transfected ceils grew vigorously and rapidly, lost contact inhibition, formed more colonies in soft agar,and developed tumors in nude mice. Conclusion：The BARF1 gene from EBV can alter the biological characteristics of HBEC by inducing cell malignant differentiation. All these findings suggest that BARF1 gene as an oncogene of EB virus may play an important role in the malignant differentiation and early tumorigenesis of HBEC.