中文摘要：

目的：采用体外孵育的血性脑脊液（BCSF）模拟蛛网膜下腔出血（SAH）刺激因素，观察SAH对人脐静脉内皮细胞（HUVECs）ICAM—1表达的影响及活血化瘀中药牛膝的有效成分蜕皮甾酮（EDS）的干预效应。方法：体外培养人脐静脉内皮细胞，通过免疫细胞化学染色和RT-PCR检测对照组、BCSF处理组和EDS治疗组HUVECs的ICAM—1表达改变。结果：与对照组相比，BCSF组HUVECs的ICAM—1免疫细胞化学染色增强，ICAM—1 mRNA表达增强（P〈0．01），EDS组弱于BCSF组。结论：BCSF刺激可引起HUVECs ICAM—1的表达上调，EDS对其具有抑制作用，可能对慢性脑血管痉挛（CVS）具治疗作用。

英文摘要：

AIM： Artificial bloody cerebrospinal fluid （BCSF） was used as pathogeny on human umbilical vein endothelial cells （HUVECs） to mimic SAH status in vivo. We tried to explore expression of ICAM-1 in HUVECs and the effect of ecdysterone （EDS）. METHODS： HUVECs were used for study. BCSF was added into the culture with or without ecdysterone （200 mg·L^-1） for 24 h, and then immunocytochemistry and RT-PCR were used to observe ICAM-1 expression in HUVECs. RESULTS： Following BCSF stimulation, both of immunocytochemistry and expression of ICAM-I mRNA increased in HUVECs. EDS could reduce the expression of ICAM-1 in HUVECs following BCSF stimulation. CONCLUSION： ICAM-I expression in HUVECs could be activated directly by BCSF and reduced by EDS. EDS is possible to be a valid candidate medicine in therapy of CVS.