中文摘要：

用以吩嗪-1-羧酸为惟一碳氮源的培养基从土壤中分离得到了96株能够降解吩嗪-1-羧酸的菌株。通过限制性内切酶酶切分析（amplified ribosomal DNA restriction analysis，ARDRA），共将其分为16种类型，对这16种类型的代表菌株16SrDNA的部分序列（500bp）进行基因测序，并将测序结果在GenBank中比对，分析其在系统发育分类学上的地位。其中，菌株V-6，204和207分属于细菌拟杆菌门的不同纲目，菌株132属于放线菌中的红球菌属，其他12株菌株分属于α、β、γ-变形菌纲。对16株代表菌株降解吩嗪-1-羧酸的能力进行了测定，结果表明，16株代表菌株能降解吩嗪-1-羧酸，但其降解效率低，培养35d后的降解率为16．5％．34．6％。降解效率最高的两株菌株为133和102．其降解率分别为34．6％和32．9％，与菌株133亲缘关系最近的菌为Pseudomonas alcaligenes ，同源性为100％；与菌株102亲缘关系最近的菌为Pseudomonas putida KT2440，同源性为99％。而将16株代表菌株人为组成混合微生物体系后，其降解效率7d即可达到26．8％，28d后达到43．8％，混合菌株对吩嗪-1-羧酸的降解效率明显优于单菌。本研究首次在环境中筛选分离到了能够降解吩嗪-1-羧酸的菌株，为进一步研究吩嗪-1-羧酸在环境中的行为提供了可能。

英文摘要：

Ninety-six bacterial strains degrading phenazine-1-carboxylic acid were isolated from soil on phenazine-1-carboxylic acid plates which were used as sole sources of carbon and energy. These isolates were analyzed by amplified ribosomal DNA restriction analysis （ARDRA） and classified into 16 types. The partial nucleotide sequences of 16S rDNA were determined and analyzed in GenBank database. Strain V-6, 204 and 207 belong to different classes of bacteroidetes, strain 132 belongs to actinobacteria, others belong to α, β, γ-proteobacteria. The degradation rates of phenazine-1-carboxylic acid by these 16 strains acting alone were relatively low, being 16.5%-34.6% after 35 days cultured. The degradation rates of strain 133 and 102 were 34.6% and 32.9% respectively. Strain 133 was identified as Pseudomonas alcaligenes, the identity was 100%; strain 102 was related to Pseudomonas putida KT2440, the identity was 99%. The mixed microbial community of these 16 strains had higher degradation rate than single strain, up to 26.8% and 43.8% after 7 days and 28 days respectively. The first isolation of phenazine-1-carboxylic acid degrading bacteria from soil samples could provide further information on the fate of phenazine-1-carboxylic acid in the environment.