以萼花臂尾轮虫(Brachionus calycbqourus)为受试动物,以17β-雌二醇(E2)为阳性对照,运用3d种群增长和4d休眠卵产量实验方法研究了不同浓度的邻苯二甲酸二丁酯(DBP)、邻苯二甲酸二异辛酯(DEHP)和邻苯二甲酸丁苄酯(BBP)对轮虫种群增长和有性生殖的影响。结果显示,与空白对照组相比,0.05—5000μg-L的E2和500μg/L的BBP显著提高了轮虫种群增长率,而0.5和5000μg/L的DBP以及0.05和0.5μg/L的BBP使轮虫种群增长率显著降低;0.5—500μg/L的E2、50和500μg/L的DBP、0.005和500μg/L的DEHP和500μg/L的BBP显著提高了轮虫的休眠卵产量,而5000μg/L的DBP和0.05μg/L的BBP却与其相反;5和500μg/L的E2、50和500μg/L的DEHP以及500μg/L的DBP和BBP均使轮虫混交率显著上升,而0.005和0.05μg/L的E2、5000μg/L的DBP和DEHP及0.5μg/L的BBP显著降低了轮虫种群中混交雌体受精率;除5000μg/L的E2显著降低轮虫种群中的混交雌体数,非混交雌体数之外,其它所有处理组均对其无显著性影响;5、500和5000μg/L的E2、5000μg/L的DBP和5—5000μg/L的DEHP均使轮虫种群中的带卵雌体数,不带卵雌体数显著升高,而BBP对其无显著影响。在实验设置浓度范围内,轮虫种群中的混交雌体数,非混交雌体数与E2浓度间、轮虫种群中的混交雌体受精率和种群增长率与DBP浓度间、轮虫休眠卵产量与DEHP浓度间、轮虫混交率与BBP浓度间均具有显著的效应一剂量关系.
The effects of Di-n-butyl phthalate (DBP), Di (2-ethylhexyl) phthalate (DEHP) and Butyl benzyl phthalate (BBP) on population growth and sexual reproduction of Brachionus calyciflorus were studied by means of 3-day population growth and 4- day resting egg production tests, and with 17β-estradiol (E2) as the positive control. Compared to the control, 0.05 -5 000 μg/L E2, and 500 μg/L BBP increased significantly the population growth rate of the rotifers, but the reverse was true for 0.5 and 5 000 μg/L DBP, and 0.05 and 0.5 μg/L BBP. 0.5 - 500 μg/L E2, 50 and 500 μg/L DBP, 0.005 and 500 μg/L DEHP, and 500 μg/ L BBP increased markedly the resting egg production of the rotifers, but the reverse was also true for 5 000 μg/ DBP and 0.05 μg/L BBP. 5 and 500 μg/L E2, 50 and 500 μg/ DEHP, and 500 μg/L DBP and BBP increased significantly the mictic rates in the rotifer populations. 0.005 and 0.05 μg/L E2, 5 000 μg/L DBP and DEHP, and 0.5 μg/L BBP decreased significantly the fertilization rate of mictic females. All compounds tested did not significantly affect the ratio of mictic females/amictic females, with the exception of 5 000/μg/L E2 which decreased markedly the ratio. 5, 500 and 5 000/μg/L E2, 5 000 μg/L DBP, and 5 - 5 000 μg/L DEHP increased significantly the ratio of ovigerous females/non-ovigerons females, but BBP had no significant effect on it. In the range of experiment concentration, significant dose-response relationships existed between the ratio of ovigerous females/ non-ovigerous females and E2 concentration, fertilization rate as well as the population growth rate and DBP concentration, resting egg production and DEHP, and mictic rate and BBP concentration.