中文摘要：

目的建立新西兰兔慢性肾病模型,应用声触诊组织定量（VTQ）技术监测兔慢性肾病的发展,为VTQ评估慢性肾病肾纤维化提供实验依据。材料与方法选取38只健康新西兰大白兔,其中32只每天经耳缘静脉注射阳离子牛血清白蛋白（C-BSA）诱导慢性肾病模型,另外6只未注射C-BSA设为对照组。于0周、2周、4周、6周、8周采用VTQ测量兔肾皮质硬度,随后解剖兔,评估肾脏的病理变化,并分析VTQ参数与病理参数的相关性。结果兔肾皮质0周、2周、4周、6周、8周的VTQ值分别为（1.68±0.25）m/s、（1.70±0.31）m/s、（1.87±0.35）m/s、（2.19±0.31）m/s、（2.46±0.46）m/s,6周VTQ值与4周比较差异有统计学意义（P〈0.05）,8周VTQ值与4周、6周比较差异有统计学意义（P〈0.05）。4周、6周、8周肾小球硬化指数分别为0.81±0.40、1.43±0.46、2.15±0.46,肾间质胶原纤维面积分别为（14.29±4.62）%、（26.28±11.06）%、（42.37±10.09）%,均明显高于0周、2周,且纤维化随病程进展逐渐增高,差异有统计学意义（P〈0.05）。肾皮质VTQ与肾小球硬化指数、肾间质胶原纤维面积呈正相关（r=0.663、0.652,P〈0.05）。结论 VTQ可以检测到兔慢性肾病发展过程中肾皮质硬度增高,与肾纤维化呈正相关,提示VTQ可以成为评价肾纤维化的重要方法。

英文摘要：

Purpose Chronic nephropathy model was established with New Zealand rabbit, and the development of chronic nephropathy was monitored using virtual touch tissue quantification （VTQ） technique, to provide the experimental basis for chronic renal fibrosis assessment using VTQ technique. Materials and Methods Thirty-eight healthy New Zealand white rabbits were selected, of which 32 were induced into chronic nephropathy model with daily intravenous ear vein injection of cationic bovine serum albumin （C-BSA）, with the other 6 rabbits without C-BSA injection as control group. At the end of 0, 2, 4, 6, 8 weeks, VTQ was used to measure the hardness of renal cortex in rabbits, then the rabbits were slaughtered to assess the pathological changes in kidney, correlation analysis between the VTQ parameters and the pathological parameters was performed. Results VTQ value of rabbit kidney cortex at 0 week, 2 weeks, 4 weeks, 6 weeks and 8 weeks was （1.68±0.25） m/s, （1.70±0.31） m/s, （1.87±0.35） m/s, （2.19±0.31） m/s and （2.46±0.46） m/s respectively, difference of VTQ values between 6 weeks and 4 weeks was statistically signiifcant （P＆lt;0.05）, and also signiifcant when compared 8 weeks with 4 weeks and 6 weeks （P＆lt;0.05）. Glomerulosclerosis index of 4 weeks, 6 weeks, 8 weeks were 0.81±0.40, 1.43±0.46 and 2.15±0.46 respectively, and tubular interstitial collagen deposition score were （14.29±4.62）%, （26.28±11.06）%, （42.37±10.09）%, which were all significantly higher than that in 0 week and 2 weeks, fibrosis increased gradually with the course, the difference was statistically signiifcant （P〈0.05）. Correlation analysis showed that the VTQ value of renal cortex was positively correlated with glomerulosclerosis index （r=0.663, P〈0.05） and tubular interstitial collagen deposition score （r=0.652, P〈0.05）. Conclusion VTQ technique is able to detect the VTQ change of renal cortex as the disease progressed in rabbits with chronic renal nephropathy, which was in