中文摘要：

对麻疯树成熟胚乳进行组织培养获得胚乳再生植株,并对其气孔进行分析.麻疯树成熟胚乳在25℃、12 h光照条件下培养7 d愈伤组织诱导完成,2,4-D浓度为2.0 mg L-1的MS培养基愈伤诱导效果最好,诱导率达89.29%.愈伤组织在含BAP的改良培养基上培养至黄绿色后转入分化培养基,在含IAA 0.25 mg L-1和ZT 1.5 mg L-1的WPM培养基上不定芽分化率达32.50%.将分化的不定芽从愈伤组织上剥离后转入含IBA、BAP和GA3的培养基上进行芽伸长培养.取胚乳不定芽叶片接种在含IBA 0.1 mg L-1、BAP 0.5 mg L-1和TDZ 0.5 mg L-1的MS培养基上诱导生芽后,再转入含IAA 0.25mgL-1、KT 0.5mg L-1、BAP 1.0 mg L-1和GA3 0.25 mg L-1的培养基上进行丛生芽的诱导,成芽率为85.2%.这些芽在含0.1 mgL-1 IBA的1/2 MS培养基上生根,大约有37.5%的芽生了根,平均有5.2条根系形成.与母本植株相比,再生的胚乳植株保卫细胞更大,且气孔密度减小.图2表6参24

英文摘要：

An effective method for endosperm culture of Jatropha curcas L.was established to develop endosperm plantlets and their stomata were analyzed.Vigorously growing calli were obtained from mature endosperm of J.curcas cultured on Murashige and Skoog（MS） medium containing 2.0 mg L-1 2,4-dichlorophenoxyacetic acid（2,4-D） at the 7th day under 25 ℃,12 h light/dark conditions,and a maximum of 89.29% of callus induction frequency was attained.The calli became kelly and had a compact shape when cultured on modified MS medium supplemented with 6-benzyladenine（BAP）.Shoot buds were produced when the calli were subcultured on a woody plant medium（WPM） with 1.5 mg L-1 zeatin（ZT） and 0.25 mg L-1 indole-3-acetic acid（IAA）,and the rate of shoot differentiation was 32.50%.The regenerated shoots were detached from the calli and transferred to the elongation medium supplemented with indole-3-butyric acid（IBA）,BAP and gibberellic acid（GA3）.The inoculated leaves from shoot buds regenerated on MS medium supplemented with 0.1 mg L-1 IBA,0.5 mg L-1 BAP and 0.5 mg L-1 thidiazuron（TDZ）.The highest shoot forming rate（85.2%） was obtained on the medium supplemented with 0.25 mg L-1 IAA,0.5 mg L-1 kinetin（KT）,1.0 mg L-1 BAP and 0.25 mg L-1 GA3.The addition of 0.1 mg L-1 IBA to the 1/2MS medium helped form roots,and approximately 37.5% of the shoots produced five roots.Stomatal analysis showed that the leaves from endosperm-derived plantlets had larger guard cells and lower density of stomata compared with their parent plantlets.Fig 2,Tab 6,Ref 24