中文摘要：

目的 了解革兰阴性杆菌的临床分布及耐药性，为临床合理用药提供依据。方法对2005年7月至12月临床分离348株革兰阴性杆菌药敏试验结果进行回顾性分析。结果 348株临床分离革兰阴性菌中最常见依次为大肠埃希菌（19．54％）、铜绿假单胞菌（12．64％）、肺炎克雷伯菌（12．07％）、不动杆菌属（11．21％），对15种临床常用抗生素不同程度耐药。产ESBLs大肠埃希菌和肺炎克雷伯菌比例为63．64％和50．00％，耐药明显。结论 我院革兰阴性杆菌存在着不同程度的耐药性，应加强细菌的耐药性监控，并强调抗生素的合理应用。

英文摘要：

Objective To investigate the distribution and drug resistance of Gram-negative bacilli and guide clinic rational administration of drugs. Methods Drug sensitivity was tested in 348 non-repeated Gram-negative strains from July to December in 2005. Results In the 348 Gram-negative isolates, Escherichia coli（ 19.54% ） was the most common isolate, followed by Pseudomonas aeruginosa （ 12. 64% ）, Klebsierlla peumoniae （ 12.07% ） and Acinetobacter （11.21% ）, these bacteria were resistant to 15 antibiotics to some degrees. E. coli and K. pheumoniae isolates producing ESBLs accounted for 63.64% and 50. 00% respectively. Conclusion Gram-negative bacilli exhibit different degrees of resistance to antimicrobial agents in our hospital. Therefore, the drug resistance of surveillance must be improved, and we must emphasize reasonable option of antimicrobial agents in clinical therapy.