中文摘要：

目的研究临床分离志贺菌产β-内酰胺酶的发生率及其基因型分布情况。方法用聚合酶链反应（PCR）方法扩增CTX-M组、OXA组、blaTEM-1、blaSHV-1、AmpC基因。PCR产物纯化测序法明确β-内酰胺酶基因型。结果91株痢疾杆菌产β-内酰胺酶的检出率为58株（63．74％），产β-内酰胺酶菌中blaTEM-1、blaCTX-M-1、blaCTX-M-13、blaOXA-1和blaAmpc扩增阳性率分别为62．07％（36／58）、17．24％（10／58）、24．14％（14／58）、36．21％（21／58）和10．34％（6／58），产AmpC中全为EBC型；携带2种耐药基因的菌株占20．88％，携带3种耐药基因的为5．49％，未测出CTX-M-2、CTX-M-8、CTX-M-25、OXA-2、OXA-10、SHV-1型。结论本地区志贺菌β-内酰胺酶的检出率较高，以TEM-1型和OXA-1型基因为主。

英文摘要：

Objective To investigate the distribution of shigella produing β-1aetamases,and the genotyes of β-1actamases among clinical shigella isolates. Methods PCR analysis was used for blaTEM-1, blashv-1, CTX-M group, OXA group and AmpC group genes present in shigella isolates. Results The β-1actamase genes were de- tected in 58（63.74 % ）strains from 91 shigella isolates. The genes of blaTEM-1 ,blaCTX-M-1 ,bla CTX-M-13, blaOXA-1 group and blaAmpc were detected in 62.07% （36/58）, 17.24% （10/58） ,24. 14% （14/58）, 36.21% （21/58） and 10. 34% （6/58）, respectively, among β-1actamases-producing isolates. The genes of EBC were detected in 100% of Ampc-producing isolates. β-1actamases-producing organisms carrying two of these genes were 20. 88% in 91 shigella isolates,5.49% strains carried 3 types of these genes. The CTX-M-2,CTX-M-8,CTX-M- 25,OXA-2.OXA-10,SHV-1 group genes were not detected. Conclusion The prevalence of β-1actamases among clinical shigella isolates was high. The β-1actamase-genes possibly are the TEM-1, OXA-1 and CTX-M types in shigella isolates in our locality.