中文摘要：

目的建立决明子中6种游离蒽醌类成分的HPLC含量测定方法,为决明子的质量评价提供参考方法。方法采用HPLC法同时测定决明子中6种游离蒽醌类成分的含量,分别为橙黄决明素,大黄酸,决明蒽醌,大黄素,大黄酚,大黄素甲醚。色谱柱为DiamonsilTMC18色谱柱（5μm,250 mm×4.6mm）;乙腈（B）-0.1%磷酸（A）梯度洗脱;流速1.0 m L/min;检测波长285 nm;柱温为室温。结果 6种游离蒽醌类成分在相应的线性范围内均呈良好的线性关系。平均回收率均在98.0%~99.6%范围内,RSD〈2.0%。结论该方法准确、灵敏、高效,可用于决明子中游离蒽醌类成分的含量测定。

英文摘要：

Objective To develop a HPLC method for simultaneous quantitative determination of six free anthraquinones in Semen Cassiae,and to provide the analysis method for the quality control of Semen Cassiae. Methods Established a simultaneous determination of six free anthraquinones in Semen Cassiae by HPLC,including chrysophanol,emodin,rhein,physcion,obtusifolin and aurantio-obtusin. The analysis was performed on a DiamonsilTMC18column（ 5μm,250 mm × 4. 6 mm） using gradient elution with the mobile phase consisting of 0. 1% orthophosphoric acid in water and acetonitrile. The flow rate was 1. 0 m L / min. The detection wavelength was set at 285 nm and at room temperature. Results All the compounds were linear（ R2 0. 999） in the range of the test concentrations,and the average recovery of the method was between 98. 0% and 99. 6%,RSD 2. 0%. Conclusion The HPLC method is accurate,precise,and efficient,which can be used to quantitatively determine the six free anthraquinones in the Semen Cassiae.