中文摘要：

【目的】阐明不同硼处理下枳橙砧木与枳壳砧木‘纽荷尔’脐橙细胞水平上的硼利用能力差异。【方法】采用水培试验,通过测定叶片中不同形态硼（自由态硼、半束缚态硼、束缚态硼）含量并分析其相互关系,研究两种砧木脐橙叶片硼形态的差异。【结果】在缺硼B0（B 0 mg.L-1）、正常硼B05（B 0.5 mg.L-1）、高硼B50（B 5.0 mg.L-1）条件下,枳橙砧木的自由态硼含量相对较低,半束缚态硼含量相对较高,枳壳砧木的正好相反;枳橙砧木在B0、B05、B50条件下的R值（半束缚态硼/自由态硼）分别为2.31、1.23和1.06,都远大于相同处理下枳壳砧木的1.80、0.57和0.82;高硼（B 5.0 mg.L-1）条件下,枳橙砧木的自由态硼和半束缚态硼分别为16.39、17.38 mg.kg-1,都远大于枳壳砧木的8.60和7.02 mg.kg-1;对于束缚态硼,枳橙砧木的脐橙正常供硼与高硼条件下分别为7.34、7.43 mg.kg-1,已没有显著差异,而枳壳砧木的则是高硼条件下最高,为9.20 mg.kg-1。【结论】与枳壳砧木相比,枳橙砧木脐橙体内硼的跨膜能力更强,细胞水平上的硼利用能力较高,在相对较低硼水平下就能满足细胞壁的需求。

英文摘要：

【Objective】To clarify the influence of boron（B） treatments on utilization ability of B in Newhall navel orange（Citrus sinensis Osbeck ＇Newhall＇）grafted on citrange [C.sinensis（L.） Osb.× Poncirus trifoliata（L.） Raf.] or trifoliate orange [Poncirus trifoliata（L.）Raf.] at cellular level.【Method】Nutrient solution culture method was used in this study.The difference of leaf B forms between the two rootstocks was analyzed according to the concentration of B forms and their relative relationship.【Result】The results showed that the free B concentration of citrange was relatively lower and the sime-bound B concentration was relatively higher in the treatments of B deficiency（B0,B 0 mg·L-1）,normal B（B05,B 0.5 mg·L-1） and high B（B50,B 5.0 mg·L-1）,on the contrary,in trifoliate orange.R value of citrange was 2.31,1.23,and 1.06 in the treatments of B0,B05,B50,respectively,which was much higher than that（1.80,0.57,0.82） of trifoliate orange in the same treatment.The free B and semi-bound B concentrations of citrange（16.39,17.38 mg·kg-1） were more higher than that of trifoliate orange（8.60 and 7.02 mg·kg-1） in the treatment of B50.Under the conditions of normal or high B,the bound B concentration of citrange was 7.34 and 7.43 mg·kg-1,respectively,there was no significant difference,while the bound B concentration of trifoliate orange was the highest（9.20 mg·kg-1） under the condition of high boron.【Conclusion】B transmembrane transport was easier in citrange-grafted plants compared to that in trifoliate orange,so B utilization ability of Newhall navel orange grafted on citrange was higher.Furthermore,B could fully bind the cell wall in citrange-grafted plants at relatively low B supply.