中文摘要：

根据NCBI中发表的大豆Rubisco小亚基基因（rbcS）序列（AF303939-1）设计特异引物,以吉农13大豆为实验材料,采用Trizol法提取叶片总RNA,通过RT-PCR克隆大豆rbcS的cDNA。将该基因与原核表达载体pET-30a（＋）连接,转化大肠杆菌E.coli BL21感受态细胞,双酶切鉴定后筛选阳性克隆,测序结果显示：rbcS全长696bp,其中开放阅读框为537bp,编码178个氨基酸;选择含大豆rbcS cDNA阳性克隆菌液IPTG诱导,经SDSPAGE分析,诱导表达出分子量为29.1kDa的融合蛋白,表达产物大小与预计理论值相符。诱导7h蛋白表达量最高,为64.15%。

英文摘要：

The specific primers were designed for rbcS gene based on the published sequence （ AF303939 - 1 ） in NCBI. Total RNA was isolated using Trizol method from the seedling leaves of soybean variety Jinong 13 and the full length eDNA of rbcS was amplified by means of reverse transcription -polymerase chain reaction（ RT -PCR）. The amplified fragment was ligated to the prokaryotic expression vector pET - 30a（ ＋ ） and transformed into E. coli BL21 comi）etent cells. The positive clones were identified by double enzyme digestion. The sequencing results indi- cated that the complete cDNA sequence of rbcS gene was 696 bp with a 537 bp ORF （ open reading frame） and en- coded 178 amino acids. A 29.1 kDa fusion protein was expressed for rbcS by IPTG induction through SDS - PAGE analysis. The size of expression product matched with anticipated theoretical value. The protein expression quantity was maximum （64.15%） at 7 hour induction.