中文摘要：

目的：观察miR-132、miR-146a在内侧颞叶癫痫（mesial temporal lobe epilepsy,MTLE）患儿及幼年大鼠模型海马组织中的表达变化。方法：收集MTLE患儿海马标本及正常对照人海马标本,Real-time PCR方法检测两组人海马组织中miR-132、miR-146a的表达改变。利用匹罗卡品诱导建立幼年大鼠MTLE模型,收集急性期（造模后2 h）、潜伏期（造模后3周）、慢性期（造模后8周）海马组织标本及相应时间点对照组大鼠海马标本,Real-time PCR方法检测各实验组大鼠海马组织中miR-132、miR-146a的表达改变。结果：miR-132、miR-146a在患儿MTLE海马组织中表达明显升高,分别为2.2±0.1和3.0±0.2,与正常对照组（1.0±0.2）比较差异有统计学意义（P〈0.05）。miR-132在MTLE大鼠模型急性期、潜伏期及慢性期表达均明显增加,分别为5.2±0.5,2.5±0.2和3.6±0.2,较正常对照组大鼠（1.0±0.1）差异有显著性（P〈0.05）;miR-146a在MTLE大鼠急性期改变不明显,在潜伏期及慢性期表达明显增加（2.8±0.2,1.8±0.2）,与对照组大鼠（1.0±0.1）比较差异有统计学意义（P〈0.05）。结论：miR-132、miR-146a在MTLE患儿及幼年大鼠模型中存在差异表达,其可能与MTLE中脑组织炎症反应的异常调控有关。

英文摘要：

Objective：To investigate the expressions changes of miR-132 and miR-146 a in hippocampal tissues in the children with mesial temporal lobe epilepsy（ MTLE） and young rats model of MTLE.Methods：Firstly,we obtained the hippocampi from children with MTLE and normal control.Real-time PCR was used to detect the expressions of miR-132 and miR-146 a.Secondly,young rats model of MTLE was induced by chloride lithium-pilocarpine.Expression changes of miR-132 and miR-146 a were monitored by Real-time PCR method in the acute,latent,and chronic stages of disease（ 2h,3 weeks and 8 weeks after induction of lithium-pilocarpine status epilepticus,respectively）,and in control hippocampal tissues corresponding to the same time points.Results：Both miR-132 and miR-146 a showed significant upregulation（ P〈0.05） in hippocampal tissues of MTLE children（ 2.2 ± 0.1 and 3.0 ± 0.2） compared to control group（ 1.0 ±0.2）.And miR-132 was upregulated significantly in the three stages（ P〈0.05） in MTLE rats model（ 5.2 ± 0.5,2.5± 0.2 and 3.6 ± 0.2,respetively） compared to their control groups（ 1.0 ± 0.1）.Though change of miR-146 a was notobviously in the acute stage,but it increased significantly（ P〈0.05） in the latent and chronic stages（ 2.8 ± 0.2 and1.8 ± 0.2） compared to control groups（ 1.0 ± 0.1）.Conclusion：There were great differences in the expression patterns of miR-132 and miR-146 a in both MTLE children and young rats model.These maybe was involved in the dysregulation of brain inflammations during MTLE development.