为了探究用腹泻鸡粪培养的蝇蛆抗菌肽对大肠杆菌感染雏鸡的疗效,试验选用艾维茵肉仔鸡120只,大肠杆菌感染致腹泻组(90只),未感染健康组(30只)。收集各组粪培养的蝇蛆,粗提抗菌肽并测定其浓度,再用腹泻鸡粪培养的蝇蛆抗菌肽和抗生素分别治疗腹泻鸡,测定各试验组腹泻鸡血液免疫细胞、肠道细菌数,治愈率。结果显示:蝇蛆抗菌肽与抗生素治疗腹泻组后,红细胞、白细胞、淋巴细胞数量均显著低于对照组(未治疗组)(P(O.01),肠道细菌数量也显著低于对照组(未治疗组)(P〈O.05)。虽然抗生素治疗鸡腹泻的效果稍微优于蝇蛆抗菌肽,但这也说明腹泻鸡粪培养的蝇蛆抗菌肽对大肠杆菌感染引起的腹泻鸡也具有治疗作用。
In order to explore the curative effect of maggot antimierobial peptides(AMPs)from diarrhea chicken faeces on E. coli infection of chickens. 120 Avain broilers were randomly divided into two groups: E. coli infection group(90)and uninfected group(30). The maggots AMPs crude were extracted and used to cure the diarrhea chicken instead of antibiotics. The blood bio chemical indicators, the number of intestinal bacteria and the cure rate of diarrhea were measured. The results showed that the number of red blood cells(RBC)were significantly reduced(P〈0.05)compared with the control group. White blood cell(WBC) and lymphocytes were significantly reduced(P〈0. 01), the number of intestinal bacteria was significantly decreased(P〈0.05). The experiments indicate that the effect of antibiotic treatment is better than AMPs and the maggot antimicrobial peptides from diarrhea chicken faeces showed good curative effect to diarrhea chicken.