应用植物解剖学、组织化学、荧光显微观察与植物化学技术相结合,研究了木立芦荟不同叶龄叶的解剖结构、叶绿素和类胡萝卜的含量、芦荟素的含量及其合成和贮藏结构的特点。结果表明:芦荟素由同化薄壁组织产生,叶绿体的基质为其合成场所。芦荟素细胞可能是芦荟素早期贮存的场所,随着芦荟素细胞的逐渐萎缩老化,维管束鞘细胞成为贮藏芦荟素的代替场所。同一植株的叶随着叶龄的增长,维管束的密度降低,芦荟素细胞占维管束横切面的百分比减小,同化薄壁组织细胞中的叶绿体逐步衰老、解体,芦荟素含量逐渐降低,但不同叶龄叶中叶绿体色素的含量与芦荟素含量问没有明显的相关性。
A multidisciplinary approach-anatomy, histochemistry, fluorescent microscope and phytochemistry was used to investigate the leaf structure, the content of chlorophyll and carotenoids,the content of aloin and the characteristics of production and storage of aloin in different leaf-age leaves Aloe arborescens. The results showed that aloin was produced in the chloroplast matrix of the assimilating tissue. Aloin was ini tially .stored in the aloin cells and then in bundle sheath cells because of aloin cells atrophy and aging. Mo- reover,the aloin content reduced gradually from top to bottom with leaf growth in the same plant. Mean while, the density of vascular bundles and the percentage of aloin cells decreased because of aloin cells atro- phy and chloroplasts aging. However, no significant correlation was found between the content of chloro- plast pigments and aloin content.