中文摘要：

采用易于回收的有机酸处理微藻细胞,以微藻Chlorella protothecoides为原料,系统研究了甲酸处理对微藻油脂提取的影响.结果表明,甲酸能有效破碎微藻细胞,最终油脂得率和脂肪酸甲酯（FAME）得率均与传统无机酸热法相当.甲酸浓度、温度和处理时间对油脂提取有显著影响.当甲酸浓度为88%,液固比为6 m L/g,100℃下处理30 min,Chlorella protothecoides油脂得率为49.2%,FAME得率高达92.1%.甲酸处理提取到的微藻油脂脂肪酸组成与无机酸热法结果无明显区别.Chlorella protothecoides油脂主要含有5种脂肪酸,其中C18∶1含量最高.实验还考察了甲酸处理对Nannochlorum sp和Nannochloropsis oceanic的适用性,结果表明,对于不同微藻原料,甲酸处理提取到的微藻油脂得率和FAME得率均与无机酸热法结果相当。

英文摘要：

Developing an advanced cell treatment technology is very important for promoting microbial oils as the feedstock for biodiesel production. The traditional inorganic acid-heat treatment process has some problems such as difficulty in recycling inorganic acid, serious environmental pollution and so on. This paper explores using organic acid which is easy to recycle, to treat cells for lipid extraction from microalgae. Effect of formic acid treatment on lipid extraction from Chlorella protothecoides was investigated systematically. The results showed that formic acid was very effective in treating microalgae cells and the total lipid yield and fatty acid methyl esters（FAME） yield were comparable with those obtained from inorganic acid-heat treatment process. It was found that formic acid concentration, temperature, treatment time and liquid/solid ratio （l/s） had a sig- nificant influence on micro-algae lipid extraction. Under the optimum condition of formic acid concentration 88%, temperature 100℃, treatment time 30 min and ratio of liquid to solid 6 ： 1, the total lipid yield and FAME yield reached 49.2% and 92. 1%, respectively. There was no obvious difference in fatty acid composition in the microbial oils obtained from organic acid treatment and inorganic acid-heat treatment process. There were 5 kinds of fatty acid in the microbial oil from Chlorella protothecoides, among which the content of C18 ： 1 was the highest. Then, the adaptation of formic acid treatment to different microalgae material was investigated. The results showed that the total lipid yield and FAME yield of Nannochlorum sp and Nannochloropsis Oceanic were comparable with those obtained from inorganic acid-heat treatment process.