多元不饱和脂肪酸(ω-3PUFAs)是具有多种生理活性的功能因子,对人体健康有重要作用。由于ω-3PUFAs大多来源于深海鱼油或藻类油脂,含量远不能达到营养品甚至药用的要求,有效富集ω-3PUFAs已成为近年来的研究热点。文章综述了利用脂肪酶选择性催化进行ω-3PUFAs富集的有关研究进展,对酶促油脂选择性水解反应,选择性转酯反应和选择性酯化反应以及不同方法组合进行ω-3PUFAs富集的反应机理以及应用进行了综述,进而对酶法生产高含量ω-3PUFAs的应用前景进行了展望。
Polyunsaturated fatty acids(ω-3PUFAs) are functional factors with many physiological activities and play important role in human health. Most ω-3PUFAs originate from deep sea fish oil or algae oil but their supply cannot meet requirements. Therefore, it is important to develop methods for ω-3PUFA enrichment. This paper reviewed current research in ω-3PUFAs enrichment via lipase selective catalysis. Mechanism and related applications of enzymatic selective hydrolysis, selective transesterification, lipid selective esterification and their combinations were introduced. The prospect of using enzymatic catalysis for high production of ω-3PUFAs was also discussed.